推荐产品
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
rat, human
种属反应性（根据同源性预测）
mouse (based on 100% sequence homology)
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... GPR56(9289)
一般描述
G-protein coupled receptor 56 (GPR56) is a ubiquitous adhesion receptor, which belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is abundantly expressed in the in thyroid gland, brain, heart, and various tumor cells. Previous studies have suggested that GPR56 may inhibit the progression and metastasis of melanomas by interaction with extracellular-matrix proteins, such as Tissue Transglutaminase. GPR56 also plays a role in regulating VEGF production and angiogenesis via a PKCα-mediated pathway. GPR56 also plays a critical role in the development of the frontal cortex; previous studies have reported that mutations in GPR56 result in disorganized cortical lamination, which is most pronounced in the frontal cortex. This condition is known as bilateral frontoparietal polymicrogyria or BFPP.
免疫原
linear peptide corresponding to the C-terminus of Mouse GPR56.
应用
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipated GPR56 from rat kidney cell lysate and visualized through western blot (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Western Blot Analysis: A representative lot of this antibody detected GPR56 from rat kidney cell lysate (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Immunocytochemistry Analysis: A representative lot of this antibody detected GPR56 from transfected HEK293 cells (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Western Blot Analysis: A representative lot of this antibody detected GPR56 from rat kidney cell lysate (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Immunocytochemistry Analysis: A representative lot of this antibody detected GPR56 from transfected HEK293 cells (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
This Anti-GPR56, C-terminal Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of GPR56, C-terminal.
质量
Evaluated by Western Blotting in Hela cell lysate.
Western Blotting Analysis: 1 µg/ml of this antibody detected GPR56 in 10 µg of Hela cell lysate.
Western Blotting Analysis: 1 µg/ml of this antibody detected GPR56 in 10 µg of Hela cell lysate.
目标描述
~ 120 kDa observed
This receptor is heavily glycosylated in cells, and thus the apparent molecular mass of the full-length receptor in most cell types is around 90-120 kDa Moreover, this receptor is prone to extensive post-translational processing and proteolysis, and thus depending on the cell type the most prominent immunoreactive bands on Western blot are usually around 45 kDa, 25 kDa and/or 15 kDa in size
This receptor is heavily glycosylated in cells, and thus the apparent molecular mass of the full-length receptor in most cell types is around 90-120 kDa Moreover, this receptor is prone to extensive post-translational processing and proteolysis, and thus depending on the cell type the most prominent immunoreactive bands on Western blot are usually around 45 kDa, 25 kDa and/or 15 kDa in size
其他说明
Concentration: Please refer to lot specific datasheet.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Ximena Barros-Álvarez et al.
Nature, 604(7907), 757-762 (2022-04-15)
Adhesion G-protein-coupled receptors (aGPCRs) are characterized by the presence of auto-proteolysing extracellular regions that are involved in cell-cell and cell-extracellular matrix interactions1. Self cleavage within the aGPCR auto-proteolysis-inducing (GAIN) domain produces two protomers-N-terminal and C-terminal fragments-that remain non-covalently attached after
Gabriel S Salzman et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(38), 10095-10100 (2017-09-07)
Adhesion G protein-coupled receptors (aGPCRs) play critical roles in diverse biological processes, including neurodevelopment and cancer progression. aGPCRs are characterized by large and diverse extracellular regions (ECRs) that are autoproteolytically cleaved from their membrane-embedded signaling domains. Although ECRs regulate receptor
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