ABS1028
Anti-GPR56, C-terminal Antibody
from rabbit, purified by affinity chromatography
别名:
GPR56 seven-transmembrane subunit, GPR56 7TM, GPR56 subunit beta, GPR56 CT, GPR56©, TM7XN1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunocytochemistry
immunoprecipitation (IP)
western blot
immunoprecipitation (IP)
western blot
Species reactivity:
rat, human
Citations:
2
Technique(s):
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
产品名称
Anti-GPR56, C-terminal Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GPR56(9289)
Application
This Anti-GPR56, C-terminal Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of GPR56, C-terminal.
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipated GPR56 from rat kidney cell lysate and visualized through western blot (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Western Blot Analysis: A representative lot of this antibody detected GPR56 from rat kidney cell lysate (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Immunocytochemistry Analysis: A representative lot of this antibody detected GPR56 from transfected HEK293 cells (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Western Blot Analysis: A representative lot of this antibody detected GPR56 from rat kidney cell lysate (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Immunocytochemistry Analysis: A representative lot of this antibody detected GPR56 from transfected HEK293 cells (Paavola, K., et al. (2011). J. Biol. Chem. 2011, 286:28914-28921.)
Analysis Note
Evaluated by Western Blotting in Hela cell lysate.
Western Blotting Analysis: 1 µg/ml of this antibody detected GPR56 in 10 µg of Hela cell lysate.
Western Blotting Analysis: 1 µg/ml of this antibody detected GPR56 in 10 µg of Hela cell lysate.
General description
G-protein coupled receptor 56 (GPR56) is a ubiquitous adhesion receptor, which belongs to the G-protein coupled receptor 2 family and LN-TM7 subfamily. It is abundantly expressed in the in thyroid gland, brain, heart, and various tumor cells. Previous studies have suggested that GPR56 may inhibit the progression and metastasis of melanomas by interaction with extracellular-matrix proteins, such as Tissue Transglutaminase. GPR56 also plays a role in regulating VEGF production and angiogenesis via a PKCα-mediated pathway. GPR56 also plays a critical role in the development of the frontal cortex; previous studies have reported that mutations in GPR56 result in disorganized cortical lamination, which is most pronounced in the frontal cortex. This condition is known as bilateral frontoparietal polymicrogyria or BFPP.
~ 120 kDa observed
This receptor is heavily glycosylated in cells, and thus the apparent molecular mass of the full-length receptor in most cell types is around 90-120 kDa Moreover, this receptor is prone to extensive post-translational processing and proteolysis, and thus depending on the cell type the most prominent immunoreactive bands on Western blot are usually around 45 kDa, 25 kDa and/or 15 kDa in size
This receptor is heavily glycosylated in cells, and thus the apparent molecular mass of the full-length receptor in most cell types is around 90-120 kDa Moreover, this receptor is prone to extensive post-translational processing and proteolysis, and thus depending on the cell type the most prominent immunoreactive bands on Western blot are usually around 45 kDa, 25 kDa and/or 15 kDa in size
Immunogen
linear peptide corresponding to the C-terminus of Mouse GPR56.
Other Notes
Concentration: Please refer to lot specific datasheet.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Ximena Barros-Álvarez et al.
Nature, 604(7907), 757-762 (2022-04-15)
Adhesion G-protein-coupled receptors (aGPCRs) are characterized by the presence of auto-proteolysing extracellular regions that are involved in cell-cell and cell-extracellular matrix interactions1. Self cleavage within the aGPCR auto-proteolysis-inducing (GAIN) domain produces two protomers-N-terminal and C-terminal fragments-that remain non-covalently attached after
Gabriel S Salzman et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(38), 10095-10100 (2017-09-07)
Adhesion G protein-coupled receptors (aGPCRs) play critical roles in diverse biological processes, including neurodevelopment and cancer progression. aGPCRs are characterized by large and diverse extracellular regions (ECRs) that are autoproteolytically cleaved from their membrane-embedded signaling domains. Although ECRs regulate receptor
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