推荐产品
生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human, mouse, porcine
技术
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... MTHFR(4524)
一般描述
Methylenetetrahydrofolate reductase or 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20; UniProt P42898) is encoded by the MTHFR gene (Gene ID 4524) in human. MTHFR catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, the predominant circulatory form of folate and carbon donor for the re-methylation of homocysteine to methionine. Defects in MTHFR cause plasma homocysteine elevation or hperhomocysteinaemia, a risk factor for cerebrovascular, peripheral vascular, and coronary heart disease. Multiple transcription start sites and alternative splicing in the MTHFR gene result in two enzyme isoforms.
特异性
Reacts with both isoforms (PMID 12370778).
免疫原
Recombinant protein corresponding to human MTHFR.
应用
Detect MTHFR using this Anti-MTHFR Antibody validated for use in Western Blotting.
Research Category
Signaling
Signaling
Research Sub Category
Signaling Neuroscience
Signaling Neuroscience
Western Blotting Analysis: A representative lot detected MTHFR in mice plasma, kidney and brain (Chew, T.W., et al. (2011). J. Nutr. 141(8):1475-1481.).
Western Blotting Analysis: A representative lot detected MTHFR in porcine liver, human lymphocytes, human fetal kidney, human adult kidney, cells transfected with MTHFR (Rozen, R. U.S. Patent 6,833,243 B2, December 21, 2004).
Western Blotting Analysis: A representative lot detected MTHFR in HepG2, mouse liver, and RAW264.7 (Mikael, L.G., and Rozen, R. (2008). Cardiovasc. Res. 80(1):151-158).
Western Blotting Analysis: A representative lot detected recombinant MTHFR protein, as well as endogenous MTHFR in human kidney (both fetal & adult), fetal human lung, and adult human aorta (Tran, P., et al. (2002). Mamm. Genome. 13(9):483-492).
Western Blotting Analysis: A representative lot detected MTHFR in porcine liver, human lymphocytes, human fetal kidney, human adult kidney, cells transfected with MTHFR (Rozen, R. U.S. Patent 6,833,243 B2, December 21, 2004).
Western Blotting Analysis: A representative lot detected MTHFR in HepG2, mouse liver, and RAW264.7 (Mikael, L.G., and Rozen, R. (2008). Cardiovasc. Res. 80(1):151-158).
Western Blotting Analysis: A representative lot detected recombinant MTHFR protein, as well as endogenous MTHFR in human kidney (both fetal & adult), fetal human lung, and adult human aorta (Tran, P., et al. (2002). Mamm. Genome. 13(9):483-492).
质量
Evaluated by Western Blotting in RAW264.7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected MTHFR in 10 µg of RAW264.7 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected MTHFR in 10 µg of RAW264.7 cell lysate.
目标描述
~70/77 kDa observed. Uncharacterized band(s) may appear in some lysates.
外形
Unpurified
Rabbit polyclonal serum with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
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