Anti-Amyloid-like protein 1 Antibody

Amyloid-like protein 1 or APLP-1 is neuronal, membrane bound specific protein that likely has a role in post-synaptic function but most research has focused on its proteolytic fragments such as ALID1 which acts as a transcriptional activator through binding the co-activator APBB1. APLP-1 fragments also interact with various G-protein coupled receptor pathways and appear to have a role in synaptic maturation during cortical development. APLP-1 also appears to regulate neurite outgrowth through binding to components of the ECM such as heparin and collagen I. Finally a special fragment called C30 is a potent enhancer of neuronal apoptosis and is a caspase specific cleavage fragment that works internally inducing apoptosis. Thus APLP-1 has roles in apoptosis, cell adhesion, endocytosis, cell signaling and neurite extension as well as some post-synaptic function as it is found clustered at post-synaptic densities in normal cells.

Immunogen is 94% identical to Bovine

Epitope: Extracellular domain

KLH-conjugated linear peptide corresponding to the extracellular domain of Human Amyloid-like protein 1.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Amyloid-like protein 1 in human cerebral cortex and thalamic tissues.

Research Category
Neuroscience

Neuroscience

Research Sub Category
Neurodegenerative Diseases

Synapse & Synaptic Biology

This Anti-Amyloid-like protein 1 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Amyloid-like protein 1.

Evaluated by Western Blotting in Human Cortex tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Amyloid-like protein 1 in 10 µg of Human Cortex tissue lysate.

~72 kDa observed. A ~90kDa band may appear in some lysates due to glycosylation: Kim. TW., et al. (1995). Brain Res Mol Brain Res. 32(1):36-44.

Antigen Affinity Purified

Purified Rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.