Anti-DISC1 Antibody

Disrupted in schizophrenia 1 protein (DISC1) is involved in the regulation of multiple aspects of embryonic and adult neurogenesis. DISC1 may play a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. DISC1 is shown to inhibit the activation of AKT-mTOR signaling upon interaction with CCDC88A. DISC1 is required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Additionally, DISC1 is suggested to regulate the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. DISC1 has been shown to participates in the Wnt-mediated neural progenitor proliferation as a positive regulator and may have a role, together with PCNT, in the microtubule network formation. DICS1 is highly expressed in the dentate gyrus of the hippocampus. It is also expressed in the temporal and parahippocampal cortices and cells of the white matter. Expression rises within the dentate gyrus and temporal cortex from the neonatal period to infancy, declines markedly in adolescence, and declines further with aging.

Recombinant protein corresponding to mouse DISC1.

Anti-DISC1 Antibody detects level of DISC1 & has been published & validated for use in DISC1.

Immunohistochemistry Analysis: A representative lot detected DISC1 in mouse hippocampus tissue (Kuroda, K., et al. (2011). Human Mole. Gene. 20(23):4666-4683).

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Evaluated by Western Blotting in mouse wild type P0 brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected DISC1 in 10 µg of mouse wild type P0 brain and mouse knock out P0 brain tissue lysate.

~ 94 kDa observed

Affinity purified

Purified rabbit polyclonal in buffer containing PBS without preservatives.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.