Anti-Complex I-75kD Antibody

Complex I-75kD (CI-75kD) is also called NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial (NDUFS1). Complex I-75kD is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), which transfers electrons from NADH to the respiratory chain, and is thought to be part of the minimal assembly required for catalysis. Complex I-75kD defects are thought to be a cause of Mitochondrial complex I deficiency (MT-C1D).

KLH-conjugated linear peptide corresponding to 15 amino acids from the internal region of human Complex I-75kD.

Anti-Complex I-75kD, Cat. No. ABN302, is a rabbit polyclonal antibody that detects Complex I-75kD and is tested for use in Western Blotting and Immunohistochemistry.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Tested Applications

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected Complex I-75kD in rat brain, human brain, and human heart tissue lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution of this antibody detected Complex I-75kD in human kidney and human heart tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Complex I-75kD in mouse brain tissue lysates.

~75 kDa observed

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.