生物来源
rabbit
质量水平
偶联物
ALEXA FLUOR™ 488
克隆
polyclonal
种属反应性
mouse, rat
制造商/商品名称
Neuro-Chrom
技术
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
运输
wet ice
靶向翻译后修饰
unmodified
一般描述
Antibodies to neuronal proteins have become critical tools for identifying neurons and discerning morphological characteristics in culture and complex tissue. While the labeling from classic histological techniques such as Golgi staining and modern molecular approaches such as GFP constructs yield excellent cytoarchitectural detail, these approaches are technically challenging and impractical for many neuroscience research needs. Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon. To achieve as complete a morphological staining as possible across all parts of neurons, Millipore has developed a polyclonal antibody blend that reacts against key somatic, nuclear, dendritic, and axonal proteins distributed across the pan-neuronal architecture that can then be detected by a single secondary antibody. This antibody cocktail has been validated in diverse methods, cell culture and immuno-histochemistry, giving researchers a convenient and specific qualitative and quantitative tool for studying neuronal morphology.
特异性
Cat. # ABN2300A4 is specific to axons (neurites), dendrites, nucleus, and the cell body of neurons.
Reactivity with other species has not been determined.
免疫原
Epitope: Whole Neuron Marker
应用
Neuro-Chrom Pan Neuronal Marker-Rabbit, Alexa488 conjugate is an antibody targeting the Pan Neuronal Marker protein, validated for use in ICC, IHC & IF.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
质量
Routinely tested Rat E18 cortex primary neurons in Immunocytochemistry.
外形
Polyclonal antibody cocktail blend conjugated to Alexa Fluor™ 488 in PBS buffer with 15mg/mL BSA and 0.05% NaN3
储存及稳定性
Maintain at 2-8°C for up to 1 year from date of receipt.
分析说明
Control
Rat E18 cortex primary neuron cell culture or mouse adult brain cryosections.
Rat E18 cortex primary neuron cell culture or mouse adult brain cryosections.
法律信息
ALEXA FLUOR is a trademark of Life Technologies
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Neurology, 87(22), 2324-2332 (2016-10-28)
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Science (New York, N.Y.), 351(6269), 173-176 (2015-12-05)
Amyloid-like protein aggregation is associated with neurodegeneration and other pathologies. The nature of the toxic aggregate species and their mechanism of action remain elusive. Here, we analyzed the compartment specificity of aggregate toxicity using artificial β-sheet proteins, as well as
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