Anti-TREM-2

Triggering receptor expressed on myeloid cells 2 (UniProt: Q9NZC2; also known as TREM-2, Triggering receptor expressed on monocytes 2) is encoded by the TREM2 gene (Gene ID: 54209) in human. TREM2 is an Ig-like V-type receptor expressed by populations of myeloid cells in various region of the central nervous system. Its expression is also reported on macrophages and dendritic cells, but not on granulocytes or monocytes. TREM2 is highly expressed in microglia, where it, along with its adaptor protein DAP12, regulates inflammatory cytokine release and phagocytosis of apoptotic neurons. TREM-2 plays a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. It forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Apolipoprotein E (ApoE) is reported to function as a high-affinity ligand for TREM-2. ApoE binding is shown to enhance the phagocytosis of apoptotic N2a cells by primary microglia cells. Greatly reduced ApoE binding is seen with Alzheimer s disease-associated TREM2 R47H mutant. TREM2 is produced with a signal peptide sequence (a.a. 1-18), the removal of which yields the mature single-transmembrane (a.a. 175-195) protein with a large extracellular region (a.a. 19-174) that contains the V-type Ig-like domain (a.a. 29-112) and a cytoplasmic tail (a.a. 196-230). The short cytoplasmic tail contains no signaling motifs and relies on its association with the immunoreceptor tyrosine-based activation motif- (ITAM-) containing TYROBP for signal transduction.

This polyclonal antibody antibody detects Triggering receptor expressed on myeloid cells 2 in several regions of human brain. It targets an epitope within 13 amino acids from the C-terminal region.

KLH-conjugated linear peptide corresponding to 13 amino acids from the C-terminal region of human TREM-2.

Anti-TREM-2, Cat. No. ABN1471, is a highly specific rabbit polyclonal antibody that targets Triggering receptor expressed on myeloid cells 2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected TREM-2 in human cerebellum, human bone marrow, human colon, and human kidney tissue.

Research Category
Neuroscience

Evaluated by Western Blotting in human cerebellum tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected TREM-2 in 10 µg of human cerebellum tissue lysate.

~27 kDa observed; 25.45 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.