Anti-NLRP12 Antibody

NLRP12 (NACHT, LRR and PYD domains-containing protein 12) is also known as Monarch-1 or PYRIN-containing APAF1-like protein 7 (PYPAF7). NLRP12 is thought to mediate activation of CASP1 (via ASC) and NF-kappa-B (via IKK). NLRP12 has been detected only in peripheral blood leukocytes, predominantly in eosinophils and granulocytes, and at lower levels in monocytes, and is regulated by nitric oxide. NLRP12 defects can cause familial cold autoinflammatory syndrome type 2 (FCAS2).

Other homologies: Mouse (91% sequence homology).

KLH-conjugated linear peptide corresponding to human NLRP12.

Anti-NLRP12 Antibody is a highly specific rabbit polyclonal antibody, that targets NLRP & has been tested in western blotting & Flow Cytometry.

Flow Cytometry Analysis: 0.5 µg from a representative lot detected NLRP12 in rat basophil leukemia (RBL).

Research Category
Signaling

Research Sub Category
Immunological Signaling

Evaluated by Western Blotting in rat basophil leukemia (RBL) cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected NLRP12 in 10 µg of rat basophil leukemia (RBL) cell lysate.

~110 kDa observed. Uniprot describes 5 isoforms produced by alternative splicing this antibody recognizes isoforms 1 through 4 at ~120 kDa, ~114 kDa, ~107 kDa, and ~102 kDa

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Rat basophil leukemia (RBL) cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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