Anti-FcRn/FCGRT Antibody

IgG receptor FcRn large subunit p51 (UniProt P55899; also known as FcRn, IgG Fc fragment receptor transporter alpha chain, Neonatal Fc receptor) is encoded by the FCGRT gene (also known as FCRN) (Gene ID 2217) in human. Transfer of passive immunity from mother to the fetus or newborn involves the transport of IgG across several epithelia. Depending on the species, IgG is transported prenatally across the placenta and yolk sac or is absorbed from colostrum and milk by the small intestine of the suckling newborn. In both cases, apical to basolateral transepithelial transport of IgG is mediated by FcRn. FcRn is a heterodimer of an alpha-chain and beta(2)-microglobulin (beta(2)m) and differs from other IgG Fc receptors in that it is structurally related to MHC class I molecules. The assembly of the FcRn alpha-chain with beta(2)m is important for both cell surface expression and pH-dependent high affinity IgG binding. Proteins endocytosed from serum are degraded in the lysosomes. However, serum albumin (SA) and IgG bind to FcRn at low pH in the endosome after endocytosis, and are transported back to the cellular surface, where they are released into the bloodstream, resulting in an extended serum half-life.

Epitope: Alpha-2/-3 domain

Linear peptide corresponding to the Alpha-2/-3 domain of human FcRn/FCGRT.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This Anti-FcRn/FCGRT Antibody is validated for use in Western Blotting for the detection of FcRn/FCGRT .

Western Blotting Analysis: A representative lot detected FcRn/FCGRT in FO-1 cells expressing Myc-hFcRn (Praetor, A., et al. (2002). J Cell Sci. 115(Pt11):2389-2397).
Western Blotting Analysis: A representative lot detected FcRn/FCGRT in hFcRn transfected MDCK cells (Praetor, A., et al. (1999). J Cell Sci. 112(Pt14):2291-2299).

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FcRn/FCGRT in 10 µg of human liver tissue lysate.

~35/45 kDa observed, with the lower band representing non- and/or less glycosylated form(s).

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.