Anti-APC8 Antibody

Cell division cycle protein 23 homolog, also known as Anaphase-promoting complex subunit 8 (APC8) or Cyclosome subunit 8, and encoded by the gene name CDC23 or ANAPC8, is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. In Drosophila, silencing of APC8 results in developmental delay and pupal lethality with elevated levels of apoptosis, high mitotic index, and delayed or blocked mitosis.

Epitope: center region

KLH-conjugated synthetic peptide corresponding to the center region of human APC8.

Detect APC8 using this Anti-APC8 Antibody validated for use in western blotting & ICC.

Immunocytochemistry Analysis: 5 μg/mL from a representative lot detected APC8 in K562 cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 2 μg/mL of this antibody detected APC8 in K562 cell lysate.

~55 kDa observed. Uncharacterized bands may be observed in some lysate(s). Uniprot describes 3 isoforms produced by alternative splicing at ~17 kDa, ~56 kDa, and ~69 kDa

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.