Anti-phospho IRF3 (ser386) Antibody

Interferon regulatory factors (IRFs) comprise a family of transcription factors that function with the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection. IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response. In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal serine and threonine residues (between 385 and 405) leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity.

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human IRF3 (ser386) near the C-terminus.

Detect IRF-3 using this rabbit polyclonal antibody, Anti-phospho IRF3 (ser386) Antibody validated for use in western blotting.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Evaluated by Western Blotting in MCF7 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected detected IRF3 (ser386) in 10 µg of Calyculin A treated MCF7 cell lysate.

~50 kDa observed

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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