生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
mouse, rat, human
技术
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... PA2G4(5036)
一般描述
Ebp1 is an RNA-binding protein involved in growth regulation. In cancer cells, this protein and has been implicated in growth inhibition and differentiation. Ebp1 is known to bind ribosomal and double-stranded RNA. It is a found in pre-ribosomal ribonucleoprotein complexes and is believed to play a role in ribosome assembly and rRNA processing. This protein is also a component of cytoplasmic bcl-2 messenger ribonucleoprotein. Through interactions with histion deacetylases, this protein can act transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes. Ebp1 also interacts with the cytoplasmic domain of the ErbB3 receptor, potentially transducing regulatory growth signals.
特异性
Recognizes Ebp-1.
免疫原
Epitope: N-terminus
Linear Peptide corresponding to the N-terminus of Ebp1.
应用
Anti-Ebp1 N-terminal Antibody is a Rabbit Polyclonal Antibody for detection of Ebp1 also known as Proliferation-associated protein 2G4 & has been validated in WB, ICC.
Immunocytochemistry:
A 1:500 dilution from a representative lot detected Ebp1 in NIH/3T3, A431, and HeLa cells.
A 1:500 dilution from a representative lot detected Ebp1 in NIH/3T3, A431, and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
Chromatin Biology
RNA Metabolism & Binding Proteins
Chromatin Biology
质量
Evaluated by Western Blotting in A431 cell lysate.
目标描述
48 kDa Observed
联系
Replaces: 07-397
外形
Unpurified
Rabbit Polyclonal Serum with 0.05% sodium azide
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
分析说明
Control
A431 Cell Lysate.
A431 Cell Lysate.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Cancer cell international, 24(1), 261-261 (2024-07-26)
Kidney Renal Clear Cell Carcinoma (KIRC) is a common malignant tumor of the urinary system, and its incidence is increasing. ERBB3 binding protein (EBP1) is upregulated in various cancers. However, the connection between EBP1 and KIRC has not been reported.
Nature communications, 11(1), 776-776 (2020-02-09)
Human Ebp1 is a member of the proliferation-associated 2G4 (PA2G4) family and plays an important role in cancer regulation. Ebp1 shares the methionine aminopeptidase (MetAP) fold and binds to mature 80S ribosomes for translational control. Here, we present a cryo-EM
Developmental biology, 386(1), 135-151 (2013-11-28)
Satellite cells are resident stem cells of skeletal muscle, supplying myoblasts for post-natal muscle growth, hypertrophy and repair. Many regulatory networks control satellite cell function, which includes EGF signalling via the ErbB family of receptors. Here we investigated the role
Molecular cell, 81(2), 304-322 (2020-12-29)
Protein synthesis must be finely tuned in the developing nervous system as the final essential step of gene expression. This study investigates the architecture of ribosomes from the neocortex during neurogenesis, revealing Ebp1 as a high-occupancy 60S peptide tunnel exit
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