ABE394
Anti-Cohesin subunit SA-2 Antibody
from rabbit, purified by affinity chromatography
别名:
Cohesin subunit SA-2, SCC3 homolog 2, Stromal antigen 2
产品名称
Anti-Cohesin subunit SA-2 Antibody, from rabbit, purified by affinity chromatography
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
equine (based on 100% sequence homology), bovine (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... STAG2(10735)
Analysis Note
Control
HeLa cell nuclear extract
HeLa cell nuclear extract
Evaluated by Western Blot in HeLa cell nuclear extract.
Western Blot Analysis: 0.2 µg/mL of this antibody detected Cohesin Subunit SA-2 in 10 µg of HeLa cell nuclear extract.
Western Blot Analysis: 0.2 µg/mL of this antibody detected Cohesin Subunit SA-2 in 10 µg of HeLa cell nuclear extract.
Application
Detect Cohesin subunit SA-2 using this Anti-Cohesin subunit SA-2 Antibody validated for use in 0.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Cohesin Subunit SA-2 in A431 and HeLa cells.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Cohesin Subunit SA-2 from HeLa cell lysate.
Immunoprecipitation Analysis: A representative lot immunoprecipitated Cohesin Subunit SA-2 from HeLa cell lysate.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Biochem/physiol Actions
Other homologies: Mouse and Rat (93%-14/15aa).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The cohesin subunit SA-2 is a part of the cohesin complex. This complex is comprised of a heterodimer between SMC1 and SMC3 proteins, as well as RAD21 and STAG proteins (STAG1, 2, or 3). These proteins form a large ring that can trap sister chromatids after DNA replication. During prophase, the cohesion complexes dissociate from the chromatin due to the phosphorylation by PLK1 (Polo-like kinase) with the exception at the centromeres where the cohesion complexes remain. Then during anaphase, the RAD21 subunit is cleaved which causes the dissociation of the complex from chromosomes, allowing chromosome separation.
~140 kDa observed. An uncharacterized band may appear at ~35 kDa in some lysates. Uncharacterized bands may appear at ~40 kDa and ~30 kDa in Immunoprecipitation.
Immunogen
KLH-conjugated linear peptide corresponding to human Cohesin Subunit SA-2.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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