Anti-dimethyl Histone H2B Antibody (Pro1)

Histone H2B is one of four histones that form a core octameric structure required for the packaging of DNA into nucleosomes, the most basic level of chromatin arrangement. Histones undergo a number of post-translational modifications (PTM) at their N- and C-terminal tails which can influence chromatin structure and stability; and consequently, transcription, and DNA replication and repair. Acetylation is a reversible PTM that involves the transfer of acetyl groups to N-terminal lysine residues by a family of histone acetyltransferase enzymes, including GNAT, Gcn5, and p300/CBP. Histone acetylation reduces the electrostatic attractions between DNA and histones, which destabilizes chromatin structure and provide transcriptional complexes, for example, with access to DNA, potentially resulting in increased gene transcription. Lysine 120 of H2B (H2BK120) undergoes acetylation (by p300/CBP) and ubiquitination. Acetylation occurs first and is required for subsequent mono-ubiquitination. H2BK120 may function as a marker of “pre-active” chromatin, and is part of a complex regulatory system involving multiple histone modifications.

Recognizes Histone H2B dimethylated at Proline 1.

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of Human dimethyl Histone H2B methylated at Pro1.

Dot Blot Specificity Analysis:
Peptide containing various unmodified and modified Histone peptides were probed with Anti-dimethyl Histone H2B (Pro1) antibody at 0.1 ug/mL.

Immunocytochemistry Analysis:
A 10 µg/mL dilution from a representative lot detected Histone H2B dimethylated at proline 1 in A431, HeLa, and NIH/3T3 cells.

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Histones

Chromatin Biology

This Anti-dimethyl Histone H2B Antibody (Pro1) is validated for use in Western Blotting and Immunocytochemistry and Dot Blot for the detection of dimethyl Histone H2B (Pro1).

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H2B dimethylated at proline 1 in 10 µg of HeLa acid extract.

~17 kDa observed

Antigen Affinity Purified

Purified rabbit Polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.