推荐产品
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
rat, mouse, human
技术
immunohistochemistry: suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... HIST1H2AG(8969)
一般描述
Histone H2A is one of four components of the core nucleosomal structure. The nucleosome represents a unit of chromatin in which DNA is wrapped around a histone octamer. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. Histone H2A, in particular, undergoes acetylation on lysine 5 by the Tip60 enzyme which may promote unfolding of chromatin and transcription. In addition, H2A is phosphorylated on serine 1 and serine 139 (H2A.X) residues which mark mitosis and gene repression, and DNA damage, respectively. Gene repression may also result from the sumoylation of H2A’s lysine 126 residue. In addition, ubiquitination of the H2A lysine 119 residue may play a role in spermatogenesis. It is the pattern of different modifications on several histone residues that alter chromatin, rather than modification of single residues.
特异性
Broad species reactivity expected based on sequence similarity.
This antibody recognizes Histone H2A at the C-terminus.
免疫原
KLH-conjugated linear peptide corresponding to the C-terminus of human Histone H2A.
应用
Immunohistchemistry Analysis: A 1:1,000 dilution from a representative lot detected Histone H2A in liver and kidney tissues.
Use Anti-Histone H2A Antibody (Rabbit Polyclonal Antibody) validated in WB, IHC to detect Histone H2A also known as Histone H2A type 1, H2A.1, Histone H2A/p.
质量
Evaluated by Western Blot in HeLa acid extract and recombinant histone proteins.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Histone H2A on 10 µg of HeLa acid extract and recombinant histone proteins.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Histone H2A on 10 µg of HeLa acid extract and recombinant histone proteins.
目标描述
~16 kDa observed
分析说明
Control
HeLa acid extract and recombinant histone proteins
HeLa acid extract and recombinant histone proteins
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
WGK
WGK 1
Louis Legoff et al.
Environment international, 152, 106472-106472 (2021-03-13)
Chlordecone (CD), also known as Kepone, is an organochlorine insecticide that has been used in banana crops in the French West Indies. Due to long-term contamination of soils and water, the population is still exposed to CD. Exposure to CD
Xiaoyu Li et al.
EMBO reports, 16(9), 1192-1202 (2015-07-24)
In the absence of pathogen attack, organisms usually suppress immune responses to reduce the negative effects of disease resistance. Monoubiquitination of histone variants at specific gene loci is crucial for gene expression, but its involvement in the regulation of plant
Donghang Cheng et al.
The Journal of biological chemistry, 295(50), 17060-17070 (2020-10-04)
CARM1 is a protein arginine methyltransferase (PRMT) that acts as a coactivator in a number of transcriptional programs. CARM1 orchestrates this coactivator activity in part by depositing the H3R17me2a histone mark in the vicinity of gene promoters that it regulates.
Xia Ting et al.
Nucleic acids research, 47(18), 9721-9740 (2019-09-11)
How chromatin dynamics is regulated to ensure efficient DNA repair remains to be understood. Here, we report that the ubiquitin-specific protease USP11 acts as a histone deubiquitinase to catalyze H2AK119 and H2BK120 deubiquitination. We showed that USP11 is physically associated
Yilei Zhang et al.
Nature cell biology, 20(10), 1181-1192 (2018-09-12)
The roles and regulatory mechanisms of ferroptosis (a non-apoptotic form of cell death) in cancer remain unclear. The tumour suppressor BRCA1-associated protein 1 (BAP1) encodes a nuclear deubiquitinating enzyme to reduce histone 2A ubiquitination (H2Aub) on chromatin. Here, integrated transcriptomic
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