Anti-ARID1B Antibody

ARID1B (AT rich interactive domain 1B), also known as BAF250B, p250R and hOsa2, is a nuclear protein and a member of the SWI/SNF-A chromatin-remodeling complex. It is involved in repression of select genes by chromatin remodeling and transcriptional activation. It non-specifically binds DNA and alters DNA nucleosome topology. The cause of mental retardation autosomal dominant type 12 (MRD12) are defects in ARID1B. High levels of ARID1B can be found in heart, skeletal muscle and kidney.

KLH-conjugated linear peptide corresponding to human ARID1B.

Immunoprecipitation Analysis: A representative lot immunoprecipitated ARID1B from Raji cell nuclear extract.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

This Anti-ARID1B Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of ARID1B.

Evaluated by Western Blot in Raji cell nuclear extract.

Western Blot Analysis: 1.0 µg/mL of this antibody detected ARID1B in 10 µg of Raji cell nuclear extract.

~250 kDa observed. Uncharacterized bands may be observed at ~16 kDa and ~32 kDa in some lysates.. Uniprot describes 4 isoforms produced by alternative splicing at ~236 kDa, ~237 kDa, ~241 kDa and ~162 kDa This antibody will recognize all isoforms.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Raji cell nuclear extract

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.