Anti-Srg3/BAF155 Antibody

SWI/SNF complex subunit SMARCC1 (UniProt P97496; also known as BAF155, BRG1-associated factor 155, SWI/SNF complex 155 kDa subunit, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 1, SWI3-related protein) is encoded by the Smarcc1 (also known as Baf155, Srg3) gene (Gene ID 20588) in murine species. SMARCC1/BAF155/SRG3 is a core component of the SWI/SNF complex that coordinates the expression of specific genes crucial for germinal center (GC) formation, including Blimp-1 and Bcl-6. The SWI/SNF is a chromatin-remodeling complex that drives conformational changes of nucleosomes in an ATP-dependent manner. SWI/SNF complex is required for the differentiation of GC B and T follicular helper (Tfh) cells. Smarcc1/Baf155/Srg3-knockout mice showed impaired differentiation of GC B and Tth cells in response to T cell-dependent antigen challenge. The SWI/SNF complex consists of at least 12 different subunits, including BRG1 with ATPase activity and SMARCC1/BAF155/SRG3 that controls the complex stability. SMARCC1/BAF155/SRG3 contains an N-terminal acidic region (23% Asp/Glu content from a.a. 211-441), a DNA-binding SWIRM domain (a.a. 448-545), a histone-interacting SANT domain (a.a. 617-668), a leucine-zipper motif (a.a. 899-942), and a C-terminal glutamine- and proline-rich region (a.a. 768-862 & 977-1104).

Reacts with both mouse and human Srg3/BAF155 proteins. Reproted to exhibit cross-reactivity toward a ~170 kDa murine ortholog of human BAF170 protein (Jeon, S.H., et al. (1997). J. Exp. Med. 185(10):1827-1836). Expected to react with also murine Srg3/Smarcc1 spliced isoform 2 reported by UniProt (UniProt P97496-2).

Epitope: C-terminal region.

GST-tagged recombinant mouse Srg3/BAF155 C-terminal fragment.

Anti-Srg3/BAF155 Antibody is an antibody against Srg3/BAF155 for use in Western Blotting, Chromatin Immunoprecipitation (ChIP).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

Western Blotting Analysis: A representative lot detected Srg3 immunoprecipitated from the nuclear extracts of human Raji and murine BAL17 B cells (Choi, J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(7):E718-727).
Western Blotting Analysis: A representative lot detected the ~155 kDa target Srg3 band and a ~170 kDa cross-reactive band in murine thymus and lymph node lysates. The ~170 kDa band likely represents the murine ortholog of human BAF170 protein (Jeon, S.H., et al. (1997). J. Exp. Med. 185(10):1827-1836).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Srg3 occupancy at the proximal promoter and BRE (Bcl-6 responsive element) regions of the Blimp-1 locus in germinal center (GC) B cells, but not in plasma B cells (PCs) (Choi, J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(7):E718-727).
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Srg3 occupacy at the Blimp-1 locus using chromatin preprations from untreated murine BAL17 B lymphoma cells, but not Brg1/Srg3-depleted BAL17 cells following siRNAs treatment (Choi, J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(7):E718-727).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Srg3 and its associated proteins from the nuclear extracts of human Raji and murine BAL17 B cells (Choi, J., et al. (2015). Proc. Natl. Acad. Sci. U. S. A. 112(7):E718-727).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated a ~195 kDa SWI2-like protein with the ~155 kDa Srg3 protein from murine thymus cell extracts (Jeon, S.H., et al. (1997). J. Exp. Med. 185(10):1827-1836).

Evaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Srg3/BAF155 in 10 µg of K562 cell lysate.

~155 kDa observed. 122.9 kDa (human and mouse SRG3) and 120.1 kDa (mouse spliced isoform 2) calculated. Uncharacterized band(s) may appear in some lysates.

Unpurified

Rabbit polyclonal antibody serum with 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

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