ABE1971-I
抗-磷酸化 TFEB（Ser142）
from rabbit, purified by affinity chromatography
别名:
Transcription factor EB, Class E basic helix-loop-helix protein 35, bHLHe35
产品名称
抗-磷酸化 TFEB（Ser142）, from rabbit, purified by affinity chromatography
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
species reactivity (predicted by homology)
bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
packaging
antibody small pack of 25 μL
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
target post-translational modification
phosphorylation (pSer142)
Quality Level
Gene Information
human ... TFEB(7942)
Analysis Note
通过蛋白质印迹法对转染的HEK293细胞裂解物进行评价。
蛋白质印迹分析：该抗体的1：500稀释液在用 FLAG标记的野生型TFEB转染的HEK293细胞的裂解物中检测到phospho TFEB（Ser142）。
蛋白质印迹分析：该抗体的1：500稀释液在用 FLAG标记的野生型TFEB转染的HEK293细胞的裂解物中检测到phospho TFEB（Ser142）。
Application
抗PGC-1αphospho TFEB (Ser142)，货号BE1971-I，是一种高度特异性的兔多克隆抗体，靶向丝氨酸 142 上磷酸化的转录因子 EB，并通过蛋白质印迹测试。
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
该兔多克隆抗体可特异性检测人细胞中丝氨酸 142 上的磷酸化转录因子 EB。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。
General description
观察值为〜65 kDa；计算值为52.87 kDa。在一些裂解液中可能会观察到未表征的条带。
转录因子EB（UniProt：P19484；又称E类碱性螺旋-环-螺旋蛋白35，bHLHe35）由人类TFEB（又称BHLHE35）基因（基因ID 7942： 7942）编码。TFEB是碱性螺旋-环-螺旋（bHLH）亮氨酸拉链转录因子，在溶酶体生物发生的调节中起主要作用。TFEB还是对饥饿转录反应的主要参与者，并通过正向调节自噬体形成和自噬体-溶酶体融合来控制自噬。TFEB在Ser142处被mTORC1和ERK磷酸化，从而阻止了其核易位。mTORC1的药理抑制作用以及饥饿和溶酶体破坏可激活TFEB核易位。与此类似，丝氨酸-苏氨酸激酶RIP1/RIPK1经证可激活ERK抑制基础自噬流，进而磷酸化Ser142处的TFEB，从而阻止参与自噬的基因产物发生TFEB介导的转录。
Immunogen
KLH 偶联的线性肽，对应于人转录因子 EB 亚型 1 的 N 端部分的磷酸丝氨酸 142 周围的15 个氨基酸。
Other Notes
浓度：请参考特定批次的数据表。
Physical form
亲和纯化
纯化的兔多克隆抗体，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。
Preparation Note
自收到之日起，在2-8°C条件下可稳定保存1年。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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