生物来源
rabbit
质量水平
抗体形式
serum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human
技术
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
ambient
靶向翻译后修饰
unmodified
基因信息
human ... CDCA8(55143)
一般描述
Borealin (UniProt: Q53HL2; also known as Cell division cycle-associated protein 8; Dasra-B; hDasra-B; Pluripotent embryonic stem cell-related gene 3 protein) is encoded by the CDCA8 (also known as PESCRG3) gene (Gene ID: 55143) in human. Borealin is a novel 31-kDa chromosomal passenger protein that is conserved among all vertebrates. It is a member of the chromosomal passenger holocomplex along with Aurora B, INCENP, and survivin. This complex play a vital role at the centromere to ensure proper chromosome alignment and segregation. Borealin is required for stability of the bipolar mitotic spindle and for kinetochore error correction. Borealin levels are reported to increase during G2/M phase and are reduced following exit from the M phase of cell cycle. Depletion of Borealin by RNAi is shown to retard mitotic progression and cause spindle T misattachment and abnormalities. Borealin-depleted cells are shown to align their chromosomes to a near normal metaphase plate, but they abruptly undergo multipolar anaphases.
特异性
This polyclonal antibody detects Borealin in nocodozole treated HeLa cells.
免疫原
A His-tagged full length human recombinant Borealin.
应用
Anti-Borealin Antibody, Cat. No. ABE1961, is a highly specific rabbit polyclonal antibody that targets Borealin and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Immunofluorescence Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).
Western Blotting Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).
Immunoprecipitation Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).
Western Blotting Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).
Immunoprecipitation Analysis: A representative lot detected Borealin in HeLa cells (Gassmann, R., et. al. (2004). J Cell Biol. 166(2):179-91).
质量
Evaluated by Western Blotting in Hela cell lysate treated with Nocodozole.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Borealin in 10 µg of Hela cell lysate treated with Nocodozole.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Borealin in 10 µg of Hela cell lysate treated with Nocodozole.
目标描述
~36 kDa observed; 31.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
其他说明
Concentration: Please refer to lot specific datasheet.
WGK
WGK 1
法规信息
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