Anti-phospho CENP-T (Ser47) Antibody

Centromere protein T (UniProt Q96BT3; also known as CENP-T, Interphase centromere complex protein 22) is encoded by the CENPT (also known as C16orf56, ICEN22) gene (Gene ID 80152) in human. Kinetochore complex-mediated chromosome interaction with microtubules from the mitotic spindle is essential for proper chromosome segregation during mitosis. The kinetochore is a macromolecular structure composed of almost 100 different proteins assembled upon the centromere in a cell cycle-regulated manner. CENP-T and 15 other chromatin proximal proteins constitute the Constitutive Centromere Associated Network (CCAN) present at centromeres throughout the cell cycle. Some CCAN proteins (e.g. CENPT/W complex and CENP-C) mediate DNA binding, while others (e.g. CENP-N) interact directly with CENP-A-containing nucleosomes. Evidence suggests that CENP-C and the CENP-T/W complex function as core kinetochore assembly factors in vertebrate cells.

Specifically reacts with the immunogen peptide, but not the corresponding non-phosphorylated sequence, by dot blot analysis.

Epitope: CENP-T pSer47

Linear peptide corresponding to a human CENP-T sequence containing phosphorylated Ser47.

Anti-phospho CENP-T (Ser47) Antibody is an antibody against phospho CENP-T for use in Immunocytochemistry, Dot Blot, Western Blotting.

Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected phospho CENP-T (Ser47) in HUVEC and A431 cells.
Dot Blot Analysis: 1.0 µg/mL from a representative lot detected the immunogen peptide, but not the corresponding non-phosphorylated sequence, by dot blot analysis.
Immunocytochemistry Analysis: A representative lot detected a closely synchronized CENP-T pSer47 phosphorylation and Hec1 immunoreactivity at the centromeres throughout HeLa cell cycle, starting at Telo/G1 and peaking at metaphase, followed by a steady deline of ~80% toward anaphase (Gascoigne, K.E., et al. (2011). Cell. 145(3):410-422).
Western Blotting Analysis: A representative lot detected the in vitro phosphorylation of recombinant CENP-T on Ser47 by recombinant CDK2/cyclin A (Gascoigne, K.E., et al. (2011). Cell. 145(3):410-422).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: 4.0 µg/mL of this antibody detected phospho CENP-T (Ser47) in HeLa cells.

60.4 kDa calculated.

Affinity purified

Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide and 30% glycerol.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.