Anti-KAT7 Antibody (HBO1 / MYST2)

Histone acetyltransferase KAT7 (EC 2.3.1.48; UniProt Q5SVQ0; also known as Histone acetyltransferase binding to ORC1, Lysine acetyltransferase 7, MOZ, MYST-2, SAS2 and TIP60 protein 2, YBF2/SAS3) is encoded by the Kat7 (also known as Hbo1, Myst2) gene (Gene ID 217127) in murine species. KAT7 is a MYST ( MOZ, Ybf2/Sas3, Sas2, and Tip60) family histone acetyltransferase (HAT) originally characterized as a HAT bound to origin recognition complex 1 (ORC1) and named HBO1. KAT7 displays histone H4-specific acetyltransferase activity with reduced activity toward histone H3. KAT7 plays an essential role in adipogenesis, embryonic development, and the survival of erythroblasts in fetal liver. KAT7 interacts with two JADE1 isoforms, JADE1S and JADE1L, that promote KAT7-mediated acetylation of nucleosomal histones. The KAT7-JADE1 complex shows cell cycle-dependent chromatin shuttling during mitosis via a mechanism linked to JADE1 phosphorylation. Nuclear JADE1S and HBO1 localization correlates with that of cell proliferation marker Ki67 and bulk histone H4 acetylation. KAT7 upregulation has been reported in some human cancers and tumor suppressor p53 expression suppresses KAT7 HAT activity.

This rabbit polyclonal antibody targets a central region sequence present in all human, murine, and rat KAT7 (HBO1/MYST2) spliced isoforms reported by UniProt (O95251, Q5SVQ0, Q810T5).

KLH-conjugated linear peptide corresponding to a central region sequence within the N-terminal half of human/mouse/rat KAT7 (HBO1/MYST2).

Anti-KAT7 (HBO1 / MYST2), Cat. No. ABE1436, is a highly specific rabbit polyclonal antibody that targets histone acetyltransferase KAT7 and has been tested in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected KAT7 (HBO1/MYST2) in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Evaluated by Western Blotting in L6 myoblast lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected KAT7 (HBO1/MYST2) in 10 µg of L6 rat myoblast lysate.

~83 kDa observed. 70.64/58.14/51.44/66.95/55.14 kDa (human isoform 1/2/3/4/5), 70.64/66.95/70.61/66.92/60.25 kDa (mouse isoform 1/2/3/4/5), and 70.51/66.82 kDa (rat isoform 1/2) calculated. Uncharacterized bands may be observed in some lysate(s).

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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