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一般描述
Sp1 was one of the first transcription factors to be characterized in mammalian cells. The Sp1 protein plays a role in the regulation of gene expression. This protein has three Cys2His2 zinc-finger motifs that have been shown to bind to the consensus sequence 5′-(G/T)GGGCGG(G/A)(G/A)(C/T)-3′ also known as a GC box. Sp1 is a ubiquitous transcription factor required for the expression of a variety of genes, such as those involved in the cell cycle and development. Interaction of the Sp1 protein with the GC box activates transcription of specific mRNAs from genes that contain functional recognition sites.
特异性
Homology to chimpanzee, canine, and rhesus monkey is 99%.
This antibody recognizes Sp1.
免疫原
GST-tagged recombinant protein corresponding to full-length human Sp1.
应用
Anti-Sp1 Antibody is a Rabbit Polyclonal Antibody for detection of Sp1 also known as Sp1 transcription factor, specificity protein 1 & has been validated in WB, IP, ICC.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Sp1 in HeLa and A431 cells.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated Sp1 from 500 µg of HeLa nuclear extract.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
质量
Evaluated by Western Blot in HeLa nuclear extract.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Sp1 on 10 µg of HeLa nuclear extract.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Sp1 on 10 µg of HeLa nuclear extract.
目标描述
95 kDa and 105 kDa
联系
Replaces: MABE550
外形
Protein A purified
Format: Purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
HeLa nuclear extract
HeLa nuclear extract
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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