ABE1348
抗组蛋白H2A.Z抗体（C-term）
from rabbit, purified by affinity chromatography
别名:
Histone H2A.Z, H2A/z
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ChIP, ELISA, ICC, WB
Citations:
6
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human
technique(s)
ChIP: suitable (ChIP-seq), ELISA: suitable, immunocytochemistry: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... H2AFZ(3015)
General description
H2A.Z，也称H2AFZ，是组蛋白H2A的一种变体，从酵母到人类细胞高度保守。像其他组蛋白变体一样，H2A.Z是由一种独特的基因编码的，产生一种略微不同的H2A类型，具有特殊的功能。H2A.Z通过稳定核小体内的组蛋白八聚体参与染色质压实。它通过创造一个更容易转录的染色质开放区域来促进基因表达。H2A.Z在整个基因组中非随机分布，主要针对中心外异染色质，但排除在无活性X染色体和细胞核外。H2A.Z与常染色质和兼性异染色质有关。DNA甲基化可以通过排除H2A.Z影响染色质结构和基因沉默效应，同样，H2A.Z也可以保护基因免受DNA甲基化的影响。因此，H2A.Z在调节异染色质沉默中起作用，并参与转录控制。
观测值〜16 kDa
Immunogen
与人组蛋白H2A.Z的近C末端区域相对应的KLH偶联的线性肽。
Application
染色质免疫沉淀（ChIP）：该抗体的代表性批次（每次反应1μg）用于使用HeLa和K562细胞的染色质进行ChIP。
ChIP-seq：该抗体的代表性批次（每次反应0.5 μg）用于免疫沉淀K562染色质以进行ChIP-seq分析。
蛋白印迹分析：该抗体的代表性批次按1:20 00稀释后在酸提取HeLa细胞裂解液中检测到组蛋白H2A.Z。
免疫细胞化学: 代表性批次的1：500稀释液在HeLa细胞中检测到组蛋白H2A.Z。
ChIP-seq：该抗体的代表性批次（每次反应0.5 μg）用于免疫沉淀K562染色质以进行ChIP-seq分析。
蛋白印迹分析：该抗体的代表性批次按1:20 00稀释后在酸提取HeLa细胞裂解液中检测到组蛋白H2A.Z。
免疫细胞化学: 代表性批次的1：500稀释液在HeLa细胞中检测到组蛋白H2A.Z。
该抗组蛋白 H2A.Z 抗体（C-端）经验证可用于蛋白质印迹、染色质免疫沉淀（ChIP）、ChIP-seq、免疫细胞化学和ELISA中组蛋白 H2A.Z（C-端）的检测。
Biochem/physiol Actions
识别组蛋白H2A.Z
预期具有广泛的反应性
Physical form
亲和纯化兔多克隆抗体，溶于含0.05%叠氮化物和0.05% ProClin 300的PBS。
Analysis Note
使用重组组蛋白通过蛋白质印迹进行了评估。
蛋白质印迹分析：该抗体的1.5 µg/mL稀释液检测到重组组蛋白H2A.Z，但未检测到组蛋白H1、H2A、H2A.X、H3或H4。
蛋白质印迹分析：该抗体的1.5 µg/mL稀释液检测到重组组蛋白H2A.Z，但未检测到组蛋白H1、H2A、H2A.X、H3或H4。
Other Notes
浓度：请参考特定批次的数据表。
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Carlotta Zampieri et al.
Molecular oncology, 16(6), 1259-1271 (2021-12-18)
Somatic inactivation of p53 (TP53) mainly occurs as missense mutations that lead to the acquisition of neomorphic mutant protein forms. p53 mutants have been postulated to exert gain-of-function (GOF) effects, including promotion of metastasis and drug tolerance, which generally contribute
Anas Reda et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology, 49(8), 1285-1295 (2024-02-17)
Creating long-lasting memories requires learning-induced changes in gene expression, which are impacted by epigenetic modifications of DNA and associated histone proteins. Post-translational modifications (PTMs) of histones are key regulators of transcription, with different PTMs producing unique effects on gene activity
Gilda Stefanelli et al.
Cell reports, 36(7), 109551-109551 (2021-08-19)
Rapid removal of histone H2A.Z from neuronal chromatin is a key step in learning-induced gene expression and memory formation, but mechanisms underlying learning-induced H2A.Z removal are unclear. Anp32e was recently identified as an H2A.Z-specific histone chaperone that removes H2A.Z from
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| ABE1348 | 04055977073195 |