Anti-FOXC1 Antibody

Forkhead box protein C1 (FOXC1) is a member of the family of forkhead transcription factors. These proteins are characterized by the forkhead domain--a 110 amino acid domain that binds to DNA molecules. FOXC1 has been implicated in the development of the eye; disruption of the FOXC1 gene has been linked to a number of glaucoma-related diseases such as Axenfeld-Rieger syndrome.

Other homologies: Rat (71% sequence homology).

KLH-conjugated linear peptide corresponding to human FOXC1.

Anti-FOXC1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXC1 also known as Forkhead box protein C1, Forkhead-related protein FKHL7, FREAC-3 & has been validated in WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blot Analysis: A representative lot of this antibody detected FOXC1 in HeLa, HEK293, THP1, and MDA-MB-468 cell lysates, and in human bone marrow tissue lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FOXC1 in HeLa cells. This antibody positively stains the nucleus, with some cytoplasm staining. This staining pattern has also been observed by an independent laboratory (Berry, F. B., et al. (2002). J Biol Chem. 277(12):10292-10297.).

Evaluated by Western Blot in THP1 cell lysate.

Western Blot Analysis: 0.025 µg/mL of this antibody detected FOXC1 in 10 µg of THP1 cell lysate. No Tween^® reagent was used in the wash buffers and in the primary and secondary antibody dilution buffers.

~75 kDa observed. Uniprot describes a molecular weight at ~57 kDa This protein may be observed at ~65 kDa in some cell lysates. (Tamimi, Y., et al. (2006). Hum Mol Genet. 15(21): 3229-3240.).

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
THP cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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