Anti-Wnt3a Antibody

Wnt-3a, also known as Protein Wnt3a, and encoded by the gene name Wnt3a and Wnt-3a, is a ligand for members of the frizzled family of seven transmembrane receptors and a member of the Wnt family. The Wnt family of consists of ligands involved in cell development, fate determination, organogenesis, and oncogenesis. Information on Wnt signaling has been inferred from genetic analysis of wingless (wg) signaling in Drosophila. The Wnt signaling cascade initiates at the cell membrane when Wnt interacts with its receptor, a member of the Frizzled (Fz) transmembrane receptor family and lipoprotein receptor–related proteins 5 and 6 (LRP-5/6). This interaction relays the signal into the interior of the cell where disheveled (dsh) is brought to the membrane and gets phosphorylated/activated. Dsh promotes the inactivation of GSK3 kinase activity, thus preventing the phosphorylation of β-Catenin and allowing it to accumulate as it avoids ubiquination and subsequent proteosome degradation. Stable β-Catenin accumulates in the cytoplasm where it interacts with lymphoid enhancer factor/T cell factor (LEF/TCF) and translocates into the nucleus. This results in activation of TCF-responsive genes which are thought to play key roles in development and cancer. Negative regulators of the pathway may include axin and the tumor suppressor APC. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Wnt-3a is expressed in dorsal portion of the neural tube (developing roof plate), and mesenchyme tissue surrounding the umbilical veins.

Linear peptide corresponding to mouse Wnt3a.

Immunocytochemistry Analysis: A representative lot detected Wnt3a in COS7 cell lysate expressing WT Wnt3a (Kishida, S., et al. (2004). Mol Cell Biol. 10:4487-501).
Western Blotting Analysis: A representative lot detected Wnt3a in Cos-7 cell lysate overexpressing Wnt-3a (Sato, A., et al. (2010). EMBO J. 29(1):41-54).

Research Category
Stem Cell Research

This Anti-Wnt3a antibody is validated for use in WB, IC for the detection of Wnt3a.

Evaluated by Western Blotting in Cos 7 overexpressing mouse cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Wnt3a in 10 µg of Cos 7 overexpressing mouse cell lysate.

~39 kDa observed. Uncharacterized band(s) may appear in some lysates.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.