推荐产品
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human
包装
antibody small pack of 25 μg
技术
flow cytometry: suitable
western blot: suitable
同位素/亚型
IgG
NCBI登记号
UniProt登记号
运输
ambient
靶向翻译后修饰
unmodified
基因信息
human ... HLA-DRB3(3125)
一般描述
HLA class II histocompatibility antigen, DR beta 3 chain (UniProt: P79483; also known as MHC class II antigen DRB3) is encoded by the HLA-DRB3 gene (Gene ID: 3125) in human. DRB3 is a single-pass type I membrane protein; that is synthesized with a signal peptide of 29 amino acids that is subsequently cleaved to generate a mature form. It also contains an Ig0like C1 type domain (aa 126-214). It is a heterodimer of an alpha and a beta subunit; also referred as MHC class II molecule. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the endoplasmic reticulum (ER) to form a heterononamer. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation. DRB3 binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells.
特异性
This rabbit polyclonal antibody detects HLA class II histocompatibility antigen, DR beta 3 chain in human cells. It targets an epitope within 15 amino acids from the N-terminal half.
免疫原
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human HLA class II histocompatibility antigen, DR beta 3 chain (HLA-DRB3).
应用
Flow Cytometry Analysis: 1 µg from a representative lot detected HLA-DRB3 in one million Raji cells.
Research Category
Inflammation & Immunology
Inflammation & Immunology
This rabbit polyclonal Anti-HLA-DRB3 Antibody, Cat. No. ABD113, is a rabbit polyclonal antibody tht detects HLA class II histocompatibility antigen, DR beta 3 chain (HLA-DRB3) and has been tested for use in Flow Cytometry and Western Blotting.
质量
Evaluated by Western Blotting in Raji cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected HLA-DRB3 in 10 µg of Raji cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected HLA-DRB3 in 10 µg of Raji cell lysate.
目标描述
~32 kDa observed; 29.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
外形
Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
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