推荐产品
生物来源
rabbit
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
human
种属反应性(根据同源性预测)
Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology)
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... CTNNBIP1(56998)
一般描述
Beta-catenin-interacting protein 1 (UniProt Q9NSA3; also known as Beta-catenin-interacting protein ICAT, Inhibitor of beta-catenin and Tcf-4) is encoded by the CTNNBIP1 (also known as ICAT) gene (Gene ID 56998) in human. Inhibitor of beta-catenin and TCF (ICAT) negatively regulates T-cell factor/lymphoid enhancer factor (TCF/LEF)–beta-catenin transcriptional activity by competing against TCF/LEF for beta-catenin binding. ICAT gene knockout leads to premature death of newborn mice with gut, kidney, and craniofacial anomalies, implicating an important role of ICAT in epithelial establishment/maintenance and neural crest cell differentiation. The motility of melanoma cells through 3D matrices in vitro relies on two interconvertible modes of movements. ICAT is reported to suppress the expression of developmentally downregulated 9 (NEDD9) of Wnt/beta-catenin signaling pathway in human Lu1205 melanoma cells, resulting in a shift from the Rac-dependent mesenchymal movement to the Rho/ROCK-dependent amoeboid motility in vitro and enhanced colonization of transplanted Lu1205 in the lungs of nude mice in vivo.
免疫原
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human ICAT.
应用
Detect ICAT/CTNNBIP1 using this rabbit Polyclonal Anti-ICAT Antibody, Cat. No. ABC939, validated for use in Immunocytochemistry, Western Blotting and Immunoprecipitation.
Immunoprecipitation Analysis: A representative lot co-immunoprecitated beta-catenin with ICAT from lysates of five human melanoma cell lines (Lu1205, WM852, Dauv-1, Mel501, and Skmel3) (Domingues, M.J., et al. (2014). Cancer Res. 74(7):1983-1995).
Western Blotting Analysis: A representative lot detected ICAT in Lu1205, Mel501, human melanoma cells, and Skmel3 (Domingues, M.J., et al. (2014). Cancer Res. 74(7):1983-1995).
Western Blotting Analysis: A representative lot detected ICAT in Lu1205, Mel501, human melanoma cells, and Skmel3 (Domingues, M.J., et al. (2014). Cancer Res. 74(7):1983-1995).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
质量
Evaluated by Immunocytochemistry in A431, HeLa, HUVEC and cells.
Immunocytochemistry Analysis: 4 µg/mL of this antibody detected ICAT in A431, HeLa, HUVEC cells.
Immunocytochemistry Analysis: 4 µg/mL of this antibody detected ICAT in A431, HeLa, HUVEC cells.
目标描述
~9 kDa calculated
外形
Protein G Purified
Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门