Anti-Rab32 Antibody

Ras-related protein Rab32 is a mitochondrion localized protein that acts as an anchoring protein for protein kinase A regulatory subunit which then plays a role in mitochondrial fission and mitochondrial dynamics of growth and recycling. Rab32 is also involved in phagosome maturation where it is recruited and serves as a lipid anchoring protein for various pathogens. Rab32 is expressed in my tissues in both the adult and the developing fetus. Rab32 is part of the Rab GTPase superfamily of GTP signaling proteins. Interestingly, some human specific pathogens such as Salmonella enterica need to replicate intracellularly and have evolved mechanisms to avoid Rab32/phagosome mediated engulfment and subsequent destruction. It appears to some species deliver an effector protein that cleaves Rab32 and thus are then free to develop intracellular vacuole-like vesicles in which to replicate and avoid destruction.

GST-tagged recombinant protein corresponding to mouse Rab32.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Rab32 Antibody is validated for use in Western Blotting and Immunoprecipitation for the detection of Rab32.

Western Blotting Analysis: A representative lot detected Rab32 in B16-f1 cell lysates (Ohbayashi, N., et al. (2012). MBoC. 23:669-678).
Western Blotting Analysis: A representative lot detected Rab32 in Melan-A cell lysates (Tamura, K., et al. (2009). MBoC. 20:2900-2908).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Rab32 in B16-f1 cell lysate (Ohbayashi, N., et al. (2012). MBoC. 23:669-678).

Evaluated by Western Blotting in Melan-A cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rab32 in 10 µg of Melan-A cell lysate.

~25 kDa observed

Unpurified

Rabbit polyclonal serum with 0.05% sodium azide.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.