Anti-BTG3 Antibody

BTG3 is a tumor suppression protein because overexpression impairs serum-induced cell cycle progression from G0/G1 to the S phase in vitro. BTG3 is often down regulated in cancer cell lines, and thus BTG3 is a target for enhanced cancer therapy. In normal cells BTG3 inhibits cell proliferation, cell invasion, and angiogenesis, and also regulates cell-cycle progression and differentiation in a variety of cell types. Developmentally BTG3 is normally expressed primarily in the brain and CNS during development where is modulates cell proliferation. In the adult expression is high in ovary, testis, prostate, thymus and lung. It is not expressed strongly in many cancers.

This antibodies immunogen is 94% identical in mouse and 92% identical in rat.

GST-tagged recombinant protein corresponding to human BTG3.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected BTG3 in human testis and human prostate tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-BTG3 Antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of BTG3.

Evaluated by Western Blotting in IMR-90 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected BTG3 in 10 µg of IMR-90 cell lysate.

~37 kDa observed. Uncharacterized bands may be observed in some lysates. The calculated molecular weight of this protein is 29 kDa, but can be observed at ~37 kDa due to alternate splice variants.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.