Anti-Olfactomedin-4/OLFM4 Antibody

OLFM4, also known as Olfactomedin-4, Antiapoptotic protein GW112, G-CSF-stimulated clone 1 protein (hGC-1), or hOLfD, and encoded by the gene OLFM4/GW112 is a multimeric protein that has been found both extracellularly, interacting with cell surface lectins and cadherin proteins, and intracellularly within the mitochondria and cytoplasm. OLFM4 is expressed during myeloid lineage development but also in found in adult prostate, bone marrow, and stomach, as well as pancreatic cancer cells. The protein appears to govern cell proliferation in pancreatic cancer cells however in leukemic cell lines it inhibits cell growth and induces cell differentiation and apoptosis. The gene is induced by retinoic acid contact and signaling is linked via the NFkappaB pathway.

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human Olfactomedin-4/OLFM4.

Anti-Olfactomedin-4/OLFM4 Antibody is an antibody against Olfactomedin-4/OLFM4 for use in western blotting & IHC.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Olfactomedin-4/OLFM4 in 10 µg of human liver tissue lysate.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Olfactomedin-4/OLFM4 in human pancreatic cancer sections and human prostate tissue.

Evaluated by Western Blotting in human small intestine tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Olfactomedin-4/OLFM4 in 10 µg of human small intestine tissue lysate.

~64 kDa observed. The calculated molecular weight of this protein is 57 kDa, but can be observed at ~64 kDa due to high glycosylation.

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.