推荐产品
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
纯化方式
affinity chromatography
种属反应性
mouse, rat, human
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... BAK1(578)
一般描述
Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.
特异性
This antibody recognizes BAK.
免疫原
KLH-conjugated linear peptide corresponding to human BAK at and around the N-terminus.
应用
Detect Bak (N-terminus) using this Anti-Bak (N-terminus) Antibody validated for use in WB, IH, IC & IP.
Western Blot (SNAP ID) Analysis: 1 - 4 µg/ml from a previous lot detected Bak on 10 µg of Hek293 cell lysate.
Immunoprecipitation Analysis: For unpurified antibodies. 10 µg from a previous lot immunoprecipitated Bak from 500 µg of NIH3T3 lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Bak in kidney tissue.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Bak in HeLa, NIH/3T3, and A431 cells.
Immunoprecipitation Analysis: For unpurified antibodies. 10 µg from a previous lot immunoprecipitated Bak from 500 µg of NIH3T3 lysate.
Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Bak in kidney tissue.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Bak in HeLa, NIH/3T3, and A431 cells.
质量
Evaluated by Western Blot in Hek293 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Bak on 10 µg of Hek293 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected Bak on 10 µg of Hek293 cell lysate.
目标描述
~ 24 kDa
联系
Replaces: 04-433
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Lindsey M Ludwig et al.
Methods in molecular biology (Clifton, N.J.), 1877, 77-91 (2018-12-12)
The BCL-2 family of proteins orchestrates a complex signaling network that governs the balance between cellular survival and death. A comprehensive understanding of the mechanistic interactions between these proteins continues to evolve in normal and malignant cells. The functional variation
Yonghua Zhuang et al.
Journal of virology, 90(17), 7684-7691 (2016-06-17)
The tumor suppressor p53 plays a critical part in determining cell fate both as a regulator of the transcription of several proapoptotic genes and through its binding interactions with Bcl-2 family proteins at mitochondria. We now demonstrate that p53 protein
Barbara Pernaute et al.
Developmental cell, 57(11), 1316-1330 (2022-05-22)
The changes that drive differentiation facilitate the emergence of abnormal cells that need to be removed before they contribute to further development or the germline. Consequently, in mice in the lead-up to gastrulation, ∼35% of embryonic cells are eliminated. This
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