Anti-Thrombospondin-2 Antibody

Thrombospondins (TSPs) are a family of homotrimeric multi-domain glycoproteins involved in modulation of cell adhesion, proliferation, and migration and have been implicated clinically in the development of atherosclerosis, angiogenesis, and oncogenesis. TSP2, a member of the thrombospondin family is a secreted protein, but is present only transiently in extracellular matrix and is rapidly internalized for degradation by fibroblasts after binding to the cell surface. It also interacts with matrix metalloproteinases (MMPs) and matrix serine proteases, such as plasminogen activator. TSP2 is reportedly controls the apoptosis-proliferation balance in endothelial cells. Although TSP2 is a potent inhibitor of angiogenesis, its N-terminal region exhibits pro-angiogenic activities mediated by beta 1 integrins. Loss of TSP2 expression is associated with local invasive behavior, tumor neovascularization, and metastasis. Decreased TSP2 expression has been correlated with malignant progression and angiogenesis in some cancers. TSP2 levels are also highly expressed in developing and healing tissues.

Other homologies: Mouse, Rat, and Bovine (93% sequence homology).

This antibody recognizes the Laminin G-like region of Thrombospondin-2.

Epitope: Laminin G-like region

KLH-conjugated linear peptide corresponding to the Laminin G-like region of human Thrombospondin-2.

Anti-Thrombospondin-2 Antibody is an antibody against Thrombospondin-2 for use in Western Blotting.

Evaluated by Western Blot in concentrated conditioned media from MG-63 cells.

Western Blot Analysis: 0.25 µg/mL of this antibody detected Thrombospondin-2 in 10 µg of concentrated conditioned media from MG-63 cells.

~180 kDa observed. The calculated molecular weight is 130 kDa, however Thrombospondin-2 has been shown to have bands at ~135 kDa and ~180 kDa in western blots (Streit, M., et al. (1999). PNAS. 96(26):14888-14893).

Control
Concentrated conditioned media from MG-63 cells

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.