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AB9986

Sigma-Aldrich

抗磷酸化BRCA2（Ser3291）抗体

from rabbit, purified by affinity chromatography

别名:

BRCA1/BRCA2-containing complex, subunit 2, Fanconi anemia group D1 protein 3, Fanconi anemia, complementation group D1, breast and ovarian cancer susceptibility gene, early onset, breast cancer 2 tumor suppressor, breast cancer 2, early onset, breast can

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rabbit

质量水平

100

抗体形式

affinity purified immunoglobulin

抗体产品类型

primary antibodies

克隆

polyclonal

纯化方式

affinity chromatography

种属反应性

rhesus macaque, horse, human

种属反应性（根据同源性预测）

rat (based on 100% sequence homology), mouse (based on 100% sequence homology), hamster (based on 100% sequence homology), cat (based on 100% sequence homology), equine (based on 100% sequence homology), canine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rhesus monkey (based on 100% sequence homology)

技术

dot blot: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

NCBI登记号

NP_000050.2

UniProt登记号

P51587

运输

wet ice

靶向翻译后修饰

phosphorylation (pSer3291)

基因信息

human ... BRCA2(675)

一般描述

BRCA2 （乳腺癌2型易感蛋白）是一种由BRCA2基因编码的肿瘤抑制蛋白（tumor suppressor protein ）。
该基因编码的蛋白在无错式双链断裂修复（error-free double-strand break repair）和同源重组中起直接作用。BRCA2 类似于肿瘤抑制基因BRCA1。BRCA2以及BRCA1 的遗传突变与增加的乳腺或卵巢癌患病风险有关。尽管RCA1 和BRCA2基因的结构差别很大，它们有一些类似的功能。两种基因产生的蛋白对受损DNA的修复必不可少，并且与RAD51基因产生的蛋白相互作用。这三种蛋白一起在维持人类基因组的稳定性方面起一定作用，并且防止出现可导致癌症的危险基因重排。

特异性

该抗体识别Ser3291周围内部结构域上及其周围的磷酸化人 BRCA2。

免疫原

KLH 偶联的线性肽，对应于Ser3291周围内部结构域上及其周围磷酸化的人 BRCA2。
表位：Ser3291周围的内部结构域

应用

免疫细胞化学分析： 一个先前批次的1：500稀释液在MCF-7细胞中检测到BRCA2。

免疫组化分析： 一个先前批次的1：100稀释液在转移性导管癌组织中检测到BRCA2。
抗磷酸化BRCA2（Ser3291）抗体是一种兔多克隆抗体，可用于检测磷酸化BRCA2（Ser3291），也称含BRCA1/BRCA2复合物亚基2&范科尼贫血组D1蛋白3， 已在 ICC、IHC、DB中进行了验证。
研究子类别
细胞周期，DNA 复制&修复

肿瘤蛋白

质量

通过斑点印迹法对磷酸化和非磷酸化BRCA2的修饰和未修饰肽进行评估。

斑点印迹分析： 2 µg/mL该抗体在磷酸化和非磷酸化BRCA2的修饰和未修饰肽中检测到BRCA2。

目标描述

约390 kDa

分析说明

对照
磷酸化和非磷酸化BRCA2的修饰和未修饰肽

其他说明

浓度：请参考批次特异性浓缩物的检验报告。

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WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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