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文件

AB9876

Sigma-Aldrich

抗Stargazin抗体

Chemicon®, from rabbit

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别名:
Stargazin-like TARP (transmembrane AMPA receptor regulatory protein)
UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rabbit

质量水平

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

种属反应性

rat, mouse

制造商/商品名称

Chemicon®

技术

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI登记号

UniProt登记号

运输

dry ice

靶向翻译后修饰

unmodified

基因信息

特异性

Stargazin [Stargazin样TARP(跨膜AMPA受体调节蛋白)]。 通过蛋白质印迹法,该抗体可识别大鼠脑Triton可溶性部分~ 35kda和~ 55kda的条带。该抗体主要与TARP γ -8反应,与γ -3和γ -4反应弱。Stargazin在海马体和小脑中强烈表达。

免疫原

来自大鼠细胞质结构域Stargazin的GST融合蛋白。

应用

研究子类别
突触&突触生物学
研究类别
神经科学
蛋白质印迹:0.1 μg/mL,大鼠脑的Triton可溶性部分。建议的稀释缓冲液是TBS-T,封闭缓冲液是3%脱脂牛奶。建议培养时间为室温下3小时过夜。
免疫组化:0.25-0.5 μg/mL,4%多聚甲醛固定组织。建议的稀释缓冲液是PBS-T。建议的透化方法是0.1% Triton X-100。建议培养时间为室温下2小时过夜。
免疫细胞化学
免疫沉淀:1-2 μg。建议反应容积为200-1000 μL。建议总蛋白浓度为2 mg/mL。建议培养时间为2-8°C下2小时。建议捕捉剂为蛋白A。

最佳工作稀释度必须由最终用户进行确定。
该抗Stargazin抗体经验证可用于IP、WB、IC、IH中Stargazin的检测。

外形

免疫亲和纯化
形式:纯化
液体形式,含130 mM Tris-HCl、87 mM甘氨酸,pH 7.4。

储存及稳定性

接收后,以未稀释的等分试样在-80°C下保存长达6个月。应避免反复冻融循环。解冻后抗体可在2-8°C下保存1-3周。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Proceedings of the National Academy of Sciences of the United States of America, 113(38), E5645-E5654 (2016-09-08)
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Behavioral sensitization to cocaine is associated with increased AMPA receptor (AMPAR) surface expression in the nucleus accumbens (NAc). This upregulation is withdrawal-dependent, as it is not detected on withdrawal day (WD) 1, but is observed on WD7-21. Its underlying mechanisms
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