biological source
rabbit
antibody form
purified antibody
clone
polyclonal
species reactivity
mouse, human, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
Analysis Note
Control
Brain tissue, brain lysate
Brain tissue, brain lysate
Evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected four isoforms of MBP on 10 μg of Mouse brain lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected four isoforms of MBP on 10 μg of Mouse brain lysates.
Application
Immunohistochemistry:
1:200 dilution of a previous lot was used by PAP or ABC on formalin fixed, paraffin-embedded human spinal cord. Pretreatment of tissue with proteolytic enzyme is not required.
Western Blot:
A dilution of 1:3000 was used in TTBS, O/N 4°C on a previous lot.
Optimal working dilutions must be determined by the end user.
1:200 dilution of a previous lot was used by PAP or ABC on formalin fixed, paraffin-embedded human spinal cord. Pretreatment of tissue with proteolytic enzyme is not required.
Western Blot:
A dilution of 1:3000 was used in TTBS, O/N 4°C on a previous lot.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers|Neurochemistry & Neurotrophins
Neuronal & Glial Markers|Neurochemistry & Neurotrophins
This Anti-Myelin Basic Protein Antibody is validated for use in IH, IH(P), WB for the detection of Myelin Basic Protein.
Biochem/physiol Actions
Suitable for labeling of oligodendrocytes and myelin of white matter in brain and spinal cord (gray matter stains less abundantly). Also stains peripheral nerves.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
General description
21, 18, 17 & 14 kDa.
The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.
Immunogen
Human myelin basic protein from brain
Other Notes
Concentration:Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Liquid in 0.05 mol/L Tris-HCl, pH 7.6 with 15 mmol/L sodium azide.
Format: Purified Ammonium Sulfate Precipitation
Format: Purified Ammonium Sulfate Precipitation
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10-13 - German Storage Class 10 to 13
法规信息
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