推荐产品
生物来源
rabbit
抗体形式
purified antibody
克隆
polyclonal
种属反应性
mouse, human, rat
制造商/商品名称
Chemicon®
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
NCBI登记号
UniProt登记号
一般描述
The classic group of Myelin basic protein (MBP) isoforms (isoforms 4 to 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non classic group of MBP isoforms (isoforms 1 to 3/Golli MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function.
特异性
Suitable for labeling of oligodendrocytes and myelin of white matter in brain and spinal cord (gray matter stains less abundantly). Also stains peripheral nerves.
免疫原
Human myelin basic protein from brain
应用
Immunohistochemistry:
1:200 dilution of a previous lot was used by PAP or ABC on formalin fixed, paraffin-embedded human spinal cord. Pretreatment of tissue with proteolytic enzyme is not required.
Western Blot:
A dilution of 1:3000 was used in TTBS, O/N 4°C on a previous lot.
Optimal working dilutions must be determined by the end user.
1:200 dilution of a previous lot was used by PAP or ABC on formalin fixed, paraffin-embedded human spinal cord. Pretreatment of tissue with proteolytic enzyme is not required.
Western Blot:
A dilution of 1:3000 was used in TTBS, O/N 4°C on a previous lot.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers|Neurochemistry & Neurotrophins
Neuronal & Glial Markers|Neurochemistry & Neurotrophins
This Anti-Myelin Basic Protein Antibody is validated for use in IH, IH(P), WB for the detection of Myelin Basic Protein.
质量
Evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected four isoforms of MBP on 10 μg of Mouse brain lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected four isoforms of MBP on 10 μg of Mouse brain lysates.
目标描述
21, 18, 17 & 14 kDa.
外形
Liquid in 0.05 mol/L Tris-HCl, pH 7.6 with 15 mmol/L sodium azide.
Format: Purified Ammonium Sulfate Precipitation
Format: Purified Ammonium Sulfate Precipitation
储存及稳定性
Stable for 1 year at 2-8ºC from date of receipt.
分析说明
Control
Brain tissue, brain lysate
Brain tissue, brain lysate
其他说明
Concentration:Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
储存分类代码
10-13 - German Storage Class 10 to 13
法规信息
新产品
Ann-Kathrin Uhde et al.
PloS one, 11(9), e0161883-e0161883 (2016-09-10)
Theiler´s murine encephalomyelitis virus (TMEV)-infection is a widely used animal model for studying demyelinating disorders, including multiple sclerosis (MS). The immunosuppressive cytokine Interleukin (IL)-10 counteracts hyperactive immune responses and critically controls immune homeostasis in infectious and autoimmune disorders. In order
Barthelemy Diouf et al.
Scientific reports, 6, 30757-30757 (2016-08-02)
A feature in patients with constitutional DNA-mismatch repair deficiency is agenesis of the corpus callosum, the cause of which has not been established. Here we report a previously unrecognized consequence of deficiency in MSH2, a protein known primarily for its
Type 2 Deiodinase Disruption in Astrocytes Results in Anxiety-Depressive-Like Behavior in Male Mice.
Barbara M L C Bocco et al.
Endocrinology, 157(9), 3682-3695 (2016-08-09)
Millions of levothyroxine-treated hypothyroid patients complain of impaired cognition despite normal TSH serum levels. This could reflect abnormalities in the type 2 deiodinase (D2)-mediated T4-to-T3 conversion, given their much greater dependence on the D2 pathway for T3 production. T3 normally
Stefanie Anderl et al.
Human molecular genetics, 20(23), 4597-4604 (2011-09-06)
Epileptic seizures, particularly infantile spasms, are often seen in infants with tuberous sclerosis complex (TSC) soon after birth. It is feared that there are long-term developmental and cognitive consequences from ongoing, frequent epilepsy. In addition, the hallmark brain pathology of
Vanessa Herder et al.
PloS one, 8(5), e62939-e62939 (2013-05-15)
Schmallenberg virus (SBV) is a recently discovered Bunyavirus associated mainly with abortions, stillbirths and malformations of the skeletal and central nervous system (CNS) in newborn ruminants. In this study, a detailed immunophenotyping of the inflammatory cells of the CNS of
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门