Anti-Kisspeptin-10

Metastasis-suppressor KISS-1 (UniProt: Q15726; also known as Kisspeptin-1) is encoded by the KISS1 (also known as PP5098) gene (Gene ID: 3814) in human. KISS1 is highly expressed in placenta and some expression is also observed in testis, pancreas, liver, small intestine. Low expression levels are also observed in the brain. Its expression is shown to increase in both early placentas and molar pregnancies and are reduced in choriocarcinoma cells. Its expression is reported to be higher levels in first trimester trophoblasts than at term of gestation. KISS-1 is a secreted metastasis suppressor that demonstrates inhibition of metastases formation in several types of cancers. It is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to generate the mature form that can also be cleaved into four chains known as Metastin (aa 68-121), Kisspeptin-14 (aa 108-121), Kisspeptin-13 (aa 109-12), and Kisspeptin-10 (112-121). Metastin acts as an endogenous ligand of GPR54, a GPCR. Activation of this receptor inhibits cell proliferation and cell migration that contributes to its tumor suppressor role. Kisspeptin-10 is reported to be a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself and increases intracellular Ca2+ levels in isolated first trimester trophoblasts. KISS1 expression is reported to be significantly reduced in human pancreatic cancer tissue and this reduced expression is associated with advanced disease. KISS1 also diminishes MMP9 expression by effecting reduced NF-kB binding to the promoter.

This rabbit polyclonal antibody detects Kisspeptin. It targets an epitope within Kisspeptin-10 at the C-terminal region.

KLH-conjugated linear peptide corresponding to 10 amino acids from the C-terminal region (Kisspeptin-10).

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Kisspeptin-10 in human placenta tissue sections

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in HL-60 cell lysate.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stable for 1 year at +2°C to +8°C from date of receipt.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

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