Anti-NAP-22 Antibody, complete sequence

Recognizes NAP-22 [Neuronal acidic protein 22, CAP-23, BASP1].

Epitope: Complete sequence

Recombinant full length rat NAP-22.

Immunoblotting: 1:1,000 using rat brain extract (colormetric; higher dilutions may be used with ECL). The antibody reacts with the 55 kDa NAP-22 protein. The suggested dilution buffer is TBS containing 0.05% Tween-20. Suggested blocking buffer is 5% non-fat dry milk. Suggested transfer membrane is PVDF-Immobilon. Suggested gel percentage is 10%. Overnight incubation with the antibody is recommended.

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Neuroregenerative Medicine

This Anti-NAP-22 Antibody, complete sequence is validated for use in WB for the detection of NAP-22.

Format: Purified

Semi-purified immunoglobulin. Liquid in TBS containing 0.05% sodium azide.

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Control
POSITIVE CONTROL: Neonatal rat brain extract. NAP-22 is heat and acid resistant. Heat or acid-treated supernatants from crude tissue or cell homogenates are good sources to analyze NAP-22 content by immunoblot.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.