产品名称
抗-Ki-67抗体, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
species reactivity (predicted by homology)
rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MKI67(4288)
mouse ... Mki67(17345)
rat ... Mki67(291234)
Analysis Note
免疫组化（石蜡）：
代表性批次数据。
该抗体以1：300的稀释度用于福尔马林固定、石蜡包埋的组织中于室温下放置10分钟。福尔马林固定组织的染色需要将组织在10 mM柠檬酸盐缓冲液，pH值6.0中煮沸10分钟，然后在室温下冷却20分钟。
代表性批次数据。
该抗体以1：300的稀释度用于福尔马林固定、石蜡包埋的组织中于室温下放置10分钟。福尔马林固定组织的染色需要将组织在10 mM柠檬酸盐缓冲液，pH值6.0中煮沸10分钟，然后在室温下冷却20分钟。
对照
阳性对照：乳腺癌，淋巴结组织，正常扁桃体。
阳性对照：乳腺癌，淋巴结组织，正常扁桃体。
Application
使用已证明可在IHC（P）&WB中起作用的抗Ki-67抗体（兔多克隆抗体）检测Ki-67。
免疫细胞化学：
使用AB9260兔 pAB对A431细胞进行共聚焦荧光分析。肌动蛋白丝用Alexa Fluor488-鬼笔环肽标记。核用DAPI染色。（核染色）。
蛋白质印迹分析：
先前批次的该抗体已用于蛋白质印迹法。
最佳工作稀释度必须由最终用户确定。
使用AB9260兔 pAB对A431细胞进行共聚焦荧光分析。肌动蛋白丝用Alexa Fluor488-鬼笔环肽标记。核用DAPI染色。（核染色）。
蛋白质印迹分析：
先前批次的该抗体已用于蛋白质印迹法。
最佳工作稀释度必须由最终用户确定。
Biochem/physiol Actions
识别Ki-67
General description
Ki67抗原是与原型细胞周期相关的核蛋白，通过在活跃细胞周期的所有阶段（G1、S、G2和M阶段）的增殖细胞来表达。在静息（G0）细胞中不表达。Ki67抗体可用于确定肿瘤中的细胞生长分数（通过免疫组织化学定量确定总静息细胞数中Ki67阳性细胞数 = Ki67指数 ）。在肿瘤组织中，预后值与氚胸苷标记指数相当。低Ki67指数与组织学低级别肿瘤之间具有很强的相关性。Ki67通常用作细胞周期和增殖的神经标志物。
预测值：在~345或395 kDa处可观察到359 kDa MW
Immunogen
来自人Ki-67的合成肽
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
在含有10 mM PBS，pH值7.4，含有BSA和叠氮化钠的的缓冲液中纯化的兔多克隆抗体。
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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