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Merck
CN

AB9220

Anti-Nanog Antibody

serum, Chemicon®

别名:

Anti-NANOG/STM1, Anti-STM1

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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生物来源

rabbit

质量水平

100

偶联物

unconjugated

抗体形式

serum

抗体产品类型

primary antibodies

克隆

polyclonal

种属反应性

mouse, human

制造商/商品名称

Chemicon®

技术

flow cytometry: suitable
western blot: suitable

输入

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

NCBI登记号

NM_024865.2

UniProt登记号

Q9H9S0

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... NANOG(79923)
mouse ... Nanog(71950)

相关类别

一般描述

39 kDa (MW 34 kDa)
NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).

免疫原

Synthetic peptide from human Nanog.

应用

Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Anti-Nanog Antibody is validated for use in FC, WB for the detection of Nanog.
Western blotting: 1:1,000-1:5,000. The antibody recognizes a protein of ~35 kDa on MBL-5 cell lysate. Additional bands of ~50 and ~60 kDa may be seen depending on sample and antibody dilution used.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.

Optimal working dilutions must be determined by the end user.

生化/生理作用

Recognizes Nanog, human.

外形

Unpurified
Rabbit Serum. Liquid.

制备说明

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
Whole cell lysate from mouse embryonic stem cells

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.
Hu, G; Kim, J; Xu, Q; Leng, Y; Orkin, SH; Elledge, SJ
Genes & Development null
Miguel Mendivil-Perez et al.
International journal of molecular sciences, 24(10) (2023-05-27)
Alzheimer's disease (AD) is a chronic neurological condition characterized by the severe loss of cholinergic neurons. Currently, the incomplete understanding of the loss of neurons has prevented curative treatments for familial AD (FAD). Therefore, modeling FAD in vitro is essential
Difei Dong et al.
Gene expression, 15(4), 153-162 (2012-07-13)
FoxA1 belongs to the fork head/winged-helix transcription factor family and participates in stimulating neuronal differentiation of pluripotent stem cells at early stages. To explore the biological roles of FoxA1 during this process, the stable expression of a GFP-FoxA1 fusion protein
Arlette A Haidar-Montes et al.
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Astrocytic differentiation is developmentally impaired in patients with childhood-onset schizophrenia (SCZ). To determine why, we used genetic gain- and loss-of-function studies to establish the contributions of differentially expressed transcriptional regulators to the defective differentiation of glial progenitor cells (GPCs) produced
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相关内容

RABBIT ANTI-NANOG, HUMAN
Human Stem Cell Systems

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

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