Anti-DARPP-32 Antibody, phosphoThr34

Recognizes DARPP-32 phosphorylated at threonine 34 .

Epitope: phosphoThr34

Synthetic peptide from DARPP-32, phosphoThr34.

Research Category
Neuroscience

Research Sub Category
Reward & Addiction

Signaling Neuroscience

This Anti-DARPP-32 Antibody, phosphoThr34 is validated for use in WB for the detection of DARPP-32.

Western blot: 1:1,000. The antibody recognizes the ~32 kD DARPP-32 protein phosphorylated at Thr34 protein in rat caudate lysate prepared from caudate slices that had been incubated in the absence and presence of forskolin. The labeling by the antibody to DARPP-32 Thr34 is markedly increased by the forskolin treatment.

Optimal working dilutions must be determined by the end user.

~32 kD

ImmunoAffinity Purified

Affinity Purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg per mL BSA with 50% glycerol.

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Cultured primary neurons, rat brain lysate and human breast adenocarcinoma

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.