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文件

AB9084

Sigma-Aldrich

抗多囊肾蛋白L抗体

serum, Chemicon®

别名:

Anti-PCL, Anti-PKD2L, Anti-PKDL, Anti-TRPP3

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rabbit

质量水平

抗体形式

serum

抗体产品类型

primary antibodies

克隆

polyclonal

种属反应性

mouse

制造商/商品名称

Chemicon®

技术

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI登记号

UniProt登记号

运输

dry ice

靶向翻译后修饰

unmodified

基因信息

mouse ... Pkd2(18764)

特异性

多囊肾蛋白L

免疫原

来自人多囊肾蛋白L1的N末端的合成肽。

应用

研究子类别
肾生理学
研究类别
代谢
蛋白质印迹:使用ECL为1:1,000。抗体与90 kDa 多囊肾蛋白L1反应。建议的封闭缓冲液为10%正常山羊血清(或与你的二抗相同的宿主),1%BSA的含0.05%吐温20的0.1 M PBS。建议的稀释缓冲液为1%正常山羊血清(或与你的二抗相同的宿主),1%BSA的含0.05%吐温20的0.1 M PBS。优选的凝胶百分比是4-12%梯度凝胶。

免疫细胞化学:1:1,000

免疫组化:使用荧光标记的二抗在2-8°C下以1:1,000过夜。建议的固定剂是0.1 M PBS中的4%多聚甲醛(一小时)。建议的透化方法是0.05%Triton X-100稀释缓冲液。建议的封闭缓冲液为10%正常山羊血清(或与你的二抗相同的宿主)和1%BSA的0.1 M PBS。建议的稀释缓冲液为1%正常山羊血清(或与你的二抗相同的宿主)和1%BSA的0.1 M PBS。

最佳工作稀释度必须由最终用户进行确定。
该抗多囊肾蛋白L抗体经过验证可用在 WB、IC、IH 中检测 多囊肾蛋白L1。

分析说明

对照
阳性对照:成年小鼠视网膜

阴性对照:成年小鼠肺

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Qiang Su et al.
Nature communications, 12(1), 4871-4871 (2021-08-13)
The heteromeric complex between PKD1L3, a member of the polycystic kidney disease (PKD) protein family, and PKD2L1, also known as TRPP2 or TRPP3, has been a prototype for mechanistic characterization of heterotetrametric TRP-like channels. Here we show that a truncated
Zuzana Tonelli Gombalová et al.
The Journal of comparative neurology, 528(15), 2523-2550 (2020-03-27)
Cerebrospinal fluid contacting neurons (CSF-cNs) represent a specific class of neurons located in close vicinity of brain ventricles and central canal. In contrast with knowledge gained from other vertebrate species, we found that vast majority of CSF-cNs in the spinal
Zongshi Lu et al.
Cell reports, 24(6), 1639-1652 (2018-08-09)
High salt intake is one independent risk factor for cardiac hypertrophy. Polycystic kidney disease 2-like 1 (PKD2L1, also called TRPP3) acts as a sour sensor in taste cells, and its possible role in the cardiovascular system is unknown. Here, we
Liang Cao et al.
Frontiers in cellular neuroscience, 16, 992520-992520 (2022-09-27)
The neural stem cells (NSCs) in the ventricular-subventricular zone of the adult mammalian spinal cord may be of great benefit for repairing spinal cord injuries. However, the sources of NSCs remain unclear. Previously, we have confirmed that cerebrospinal fluid-contacting neurons
Fang Sun et al.
Diabetes, metabolic syndrome and obesity : targets and therapy, 13, 1349-1358 (2020-05-20)
High-sensitivity C-reactive protein (hs-CRP) has been regarded as a biomarker of low-degree inflammation in illness; however, whether CRP exerts its pathogenic effect on the cardiometabolic system remains unknown. Aside from the beneficial effects of metabolic surgery on cardiometabolic system, its impact

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