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主要文件

AB9082

Sigma-Aldrich

抗雷诺丁受体3抗体

serum, Chemicon®

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rabbit

质量水平

抗体形式

serum

抗体产品类型

primary antibodies

克隆

polyclonal

种属反应性

rat, mouse, human

制造商/商品名称

Chemicon®

技术

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI登记号

UniProt登记号

运输

dry ice

靶向翻译后修饰

unmodified

基因信息

human ... RYR3(6263)

特异性

雷诺丁受体3。

免疫原

来自人雷诺丁受体3变体TM区的合成肽。

应用

研究子类别
离子通道&转运蛋白

信号传导神经科学
研究类别
神经科学
蛋白质印迹:使用ECL为1:1,000。该抗体与500 kDa雷诺丁受体3蛋白反应。建议的封闭缓冲液为10%正常山羊血清(或与你的二抗相同的宿主),1%BSA的含0.05%吐温20的0.1 M PBS。建议的稀释缓冲液为1%正常山羊血清(或与你的二抗相同的宿主),1%BSA的含0.05%吐温20的0.1 M PBS。优选的凝胶百分比是4-12%梯度凝胶。

免疫细胞化学:1:1,000

免疫组化:使用荧光标记的二抗在2-8°C下以1:1,000过夜。建议的固定剂是0.1 M PBS中的4%多聚甲醛(一小时)。建议的透化方法是0.05%Triton X-100稀释缓冲液。建议的封闭缓冲液为10%正常山羊血清(或与你的二抗相同的宿主)和1%BSA的0.1 M PBS。建议稀释缓冲液为含1%正常山羊血清(或与二抗相同的宿主)和1%BSA的0.1 M PBS。最佳工作稀释度必须由最终用户确定。
该抗雷诺丁受体3抗体经过验证可用在IC、IH、WB 中检测雷诺丁受体3。

外形

兔血清。 液体。

储存及稳定性

自收到之日起,在 -20°C 以未稀释的等分试样可保存 6 个月。应避免反复冻/融循环。

分析说明

对照
蛋白质印迹=成年小鼠脑(成年小鼠骨骼肌为阴性)。

IHC=成年小鼠小脑浦肯野细胞(成年小鼠小脑颗粒细胞为阴性)。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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