产品名称
Anti-p73 Antibody, α and β, Chemicon®, from rabbit
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HSPA8(3312)
Application
Anti-p73 Antibody, α & β is a Rabbit Polyclonal Antibody for detection of p73 also known as p53-like Transcription Factor & has been validated in IP, WB, IHC(P).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Western blot: 1 μg/mL of the antibody will detect p73 in 30μg of total cell lysate from overexpressing cell lines. Higher levels of the antibody may be necessary when using native tissue sources.
Immunoprecipitation: Add 1μg of antibody to a sample that has been precleared with Protein A Sepharose™ in 500 μl lysis buffer. Incubate antibody with sample at 4°C for 2 hours with agitation. Follow by incubation with 50 μL Protein A Sepharose for 2 hours at 4°C with agitation. Collect the Protein A complex by centrifugation, washing pellet 3 times in lysis buffer. Resuspend final sample in electrophesis buffer, denature by heating at 95°C for 5 minutes, and analyze by SDS-PAGE
Immunohistochemistry of paraffin sections: Signal amplification may be necessary, in conjunction with standard IHC procedures. Dewax and rehydrate sections through descending graded alcohols to water using standard dewaxing protocols. Subject section to heat antigen retrieval. Wash sections 3 times with TBS, pH 7.4. Block endogenous peroxidase activity by incubating the section in 1% H2O2 in methanol for 30 minutes. Wash with TBS and detect according to preferred detection methods. With high sensitivity detection systems, typical working dilutions for the p73 antibody are 1:5,000 - 1;10,000.
Optimal working dilutions must be determined by end user.
Immunoprecipitation: Add 1μg of antibody to a sample that has been precleared with Protein A Sepharose™ in 500 μl lysis buffer. Incubate antibody with sample at 4°C for 2 hours with agitation. Follow by incubation with 50 μL Protein A Sepharose for 2 hours at 4°C with agitation. Collect the Protein A complex by centrifugation, washing pellet 3 times in lysis buffer. Resuspend final sample in electrophesis buffer, denature by heating at 95°C for 5 minutes, and analyze by SDS-PAGE
Immunohistochemistry of paraffin sections: Signal amplification may be necessary, in conjunction with standard IHC procedures. Dewax and rehydrate sections through descending graded alcohols to water using standard dewaxing protocols. Subject section to heat antigen retrieval. Wash sections 3 times with TBS, pH 7.4. Block endogenous peroxidase activity by incubating the section in 1% H2O2 in methanol for 30 minutes. Wash with TBS and detect according to preferred detection methods. With high sensitivity detection systems, typical working dilutions for the p73 antibody are 1:5,000 - 1;10,000.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Antibody AB7824 recognizes amino acids 450-636 of p73 protein. This region is common to both p73alpha and p73beta isoforms, hence both isoforms of the p73 protein will be detected.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Epitope: alpha and beta
GST fusion protein containing aa450-636 of p73 protein.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Purified immunoglobulin. 0.22 micron filtered. Provided at 1 mg/mL in PBS, pH 7.4, containing 0.05% sodium azide.
Preparation Note
Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Julian M Rozenberg et al.
Cells, 10(1) (2020-12-31)
Epithelial organs are the first barrier against microorganisms and genotoxic stress, in which the p53 family members p63 and p73 have both overlapping and distinct functions. Intriguingly, p73 displays a very specific localization to basal epithelial cells in human tissues
T J Grob et al.
Cell death and differentiation, 8(12), 1213-1223 (2001-12-26)
Inactivation of the tumour suppressor p53 is the most common defect in cancer cells. p53 is a sequence specific transcription factor that is activated in response to various forms of genotoxic stress to induce cell cycle arrest and apoptosis. Induction
Linda Sooman et al.
Acta oncologica (Stockholm, Sweden), 54(3), 385-394 (2014-09-30)
The survival of high-grade glioma patients is poor and the treatment of these patients can cause severe side effects. This fosters the necessity to identify prognostic biomarkers, in order to optimize treatment and diminish unnecessary suffering of patients. The aim
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