AB758B
Anti-Collagen Type I Antibody, biotin conjugated
Chemicon®, from goat
别名:
Anti-CAFYD, Anti-EDSARTH1, Anti-EDSC, Anti-OI1, Anti-OI2, Anti-OI3, Anti-OI4
产品名称
Anti-Collagen Type I Antibody, biotin conjugated, Chemicon®, from goat
biological source
goat
conjugate
biotin conjugate
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
bovine, human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
dot blot: suitable
immunohistochemistry: suitable
western blot: suitable
suitability
not suitable for immunohistochemistry (Paraffin)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... COL1A1(1277)
Application
Anti-Collagen Type I Antibody, biotin conjugated is an antibody against Collagen Type Id for use in DB, ELISA, WB, IH.
Dot and slot blotting: 1:500-1:1,000
ELISA: 1:1,000-4,000
Indirect immunohistochemistry (frozen sections only): 1:20-1:40
The non-biotinylated version (Cat. # AB758) reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts , and has been cited for use in WB applications (PMID: 20089870)
Not recommended for paraffin tissue sections.
Optimal working dilutions must be determined by the end user.
ELISA: 1:1,000-4,000
Indirect immunohistochemistry (frozen sections only): 1:20-1:40
The non-biotinylated version (Cat. # AB758) reacts with human collagen I in reduced westerns with supernatant samples from serum-free cultures of human fibroblasts , and has been cited for use in WB applications (PMID: 20089870)
Not recommended for paraffin tissue sections.
Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
ECM Proteins
ECM Proteins
Biochem/physiol Actions
Human type I collagen as demonstrated by ELISA. Less than 10% cross reactivity with collagen types II, III, IV, V, and VI. May show reactivity to type I collagen from other species. AB758B has not been tested with other extracellular matrix proteins (e.g., laminin, fibronectin).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunogen
Human placental collagen type I
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Affinity purified immunoglobulin, biotin labeled. Prior to purification the antisera was adsorbed against collagen type II, III, IV, V and VI immobilized on Sepharose™ 4B.. Liquid in phosphate buffered saline, pH 7.4, containing 0.1% sodium azide.
Preparation Note
Maintain at 2-8°C in undiluted aliquots for up to 12 months.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Interleukin-6 production in CD40-engaged fibrocytes in thyroid-associated ophthalmopathy: involvement of Akt and NF-?B.
Gillespie, EF; Raychaudhuri, N; Papageorgiou, KI; Atkins, SJ; Lu, Y; Charara, LK; Mester et al.
Investigative Ophthalmology & Visual Science null
Stephen J Atkins et al.
Endocrinology, 156(12), 4731-4740 (2015-09-22)
A relationship between the actions of TSH and IGF-1 was first recognized several decades ago. The close physical and functional associations between their respective receptors (TSHR and IGF-1R) has been described more recently in thyroid epithelium and human orbital fibroblasts
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