产品名称
抗I型胶原抗体, Chemicon®, from rabbit
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
bovine
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable
suitability
not suitable for Western blot
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... COL1A1(1277)
Application
免疫组化： 1：40稀释用于冷冻牛肝和皮肤组织的免疫荧光染色。
在2%福尔马林或PFA固定，石蜡包埋的组织上进行免疫组化需要HEIR抗原修复和增强的酶法检测；甲基卡诺、丙酮或酸醛固定可增强石蜡切片反应性。
放射免疫测定
ELISA：1：200定向
不推荐用于蛋白质印迹。
在2%福尔马林或PFA固定，石蜡包埋的组织上进行免疫组化需要HEIR抗原修复和增强的酶法检测；甲基卡诺、丙酮或酸醛固定可增强石蜡切片反应性。
放射免疫测定
ELISA：1：200定向
不推荐用于蛋白质印迹。
抗I型胶原抗体可检测I型胶原水平&已发表&经验证可用于ELISA、RIA、IH、IH（P）。
研究子类别
ECM 蛋白
ECM 蛋白
研究类别
细胞结构
细胞结构
Biochem/physiol Actions
与牛I型胶原反应。
在1：500稀释的RIA中，抗体与II型，IX &XI型牛胶原或纤连蛋白的交叉反应性低于0.1%；其他胶原蛋白和ECM蛋白未经测试。
与人I型胶原的反应性<2.0%，RIA，1：500；通过RIA与I型鸡胶原的反应性在1：500稀释度下小于0.1%。
在1：500稀释的RIA中，抗体与II型，IX &XI型牛胶原或纤连蛋白的交叉反应性低于0.1%；其他胶原蛋白和ECM蛋白未经测试。
与人I型胶原的反应性<2.0%，RIA，1：500；通过RIA与I型鸡胶原的反应性在1：500稀释度下小于0.1%。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
从纯化的牛皮肤中提取的纯化的牛I型胶原。
Other Notes
替代品：AB749
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
形式：纯化
蛋白G纯化的IgG部分，溶于0.01 M磷酸盐，0.09 M NaCl，pH值7.2中1 mg/mL的液体。无防腐剂。
Preparation Note
在-20°C下可冷冻保存长达12个月。 应避免反复冻/融循环
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
未找到合适的产品？
试试我们的产品选型工具.
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Meng Wu et al.
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 25(3), 822-829 (2019-04-27)
Tibetan mural samples from the Jiazhaer mountain cave were studied using enzyme-linked immunosorbent assays (ELISA) and immunofluorescence microscopy (IFM). Samples containing protein binders were first identified using ELISA, and then IFM was used to determine the location of protein binders.
Lucio Díaz-Flores et al.
Histology and histopathology, 36(2), 217-228 (2020-12-19)
Intussusceptive angiogenesis (IA) is the process by which pre-existing blood vessels split, expand and remodel through intravascular pillar formation. In previous works, we studied the morphologic characteristics of intravascular papillary endothelial hyperplasia (IPEH) and suggested the participation of IA in
Cemile Bektas et al.
Bioengineering (Basel, Switzerland), 11(8) (2024-08-31)
Cultivated meat, an advancement in cellular agriculture, holds promise in addressing environmental, ethical, and health challenges associated with traditional meat production. Utilizing tissue engineering principles, cultivated meat production employs biomaterials and technologies to create cell-based structures by introducing cells into
Sun H Peck et al.
Cartilage, 12(4), 512-525 (2019-04-12)
Skeletal tissues such as intervertebral disc and articular cartilage possess limited innate potential to regenerate, in part due to their avascularity and low cell density. Despite recent advances in mesenchymal stem cell (MSC)-based disc and cartilage regeneration, key challenges remain
Sarah E Gullbrand et al.
Acta biomaterialia, 70, 154-164 (2018-02-11)
Replacement of the intervertebral disc with a viable, tissue-engineered construct that mimics native tissue structure and function is an attractive alternative to fusion or mechanical arthroplasty for the treatment of disc pathology. While a number of engineered discs have been
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持