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生物来源
rabbit
质量水平
抗体形式
fractionated antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
种属反应性
human
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunofluorescence: suitable
适用性
not suitable for Western blot
not suitable for immunohistochemistry (Paraffin)
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... COL3A1(1281)
特异性
The antibody reacts with native and heat denatured (non-reduced) human collagen type III. Cross reactions with other types of collagen under native conditions do occur. There is 10% cross reactivity with human collagen type I, 2% cross reactivity with human collagen type II, and 4% cross reactivity with human collagen type IV and V. There is no cross reactivity with other human plasma proteins under native ELISA conditions. Reactivity under denatured conditions has not been examined.
Cross Reactivity Percent
Human collagen type I 10%
Human collagen type II 2%
Human collagen type III 100%
Human collagen type IV and V 4%
Human plasma proteins do not interfere with binding to collagen.
Cross Reactivity Percent
Human collagen type I 10%
Human collagen type II 2%
Human collagen type III 100%
Human collagen type IV and V 4%
Human plasma proteins do not interfere with binding to collagen.
免疫原
Human placental collagen type III
应用
ELISA 1:3,000
Indirect immunofluorescence on cryostat sections or cultured cells 1:20-1:40
Optimal working dilutions must be determined by the end user.
Indirect immunofluorescence on cryostat sections or cultured cells 1:20-1:40
Optimal working dilutions must be determined by the end user.
This Anti-Collagen Type III Antibody is validated for use in ELISA, IF for the detection of Collagen Type III.
外形
500ul containing of 0.15M NaCl, 10 mM sodium phosphate pH 7.5, with 0.1% mannitol and o.1% dextran as stabilizers contains no preservatives.
Immunoglobulin fraction was prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiserum cross-absorbed over immobilized human serum proteins, immunoglobulins, and collagen types I, II, IV and V.
Immunoglobulin fraction was prepared by ammonium sulfate precipitation and chromatography on DEAE-cellulose, of antiserum cross-absorbed over immobilized human serum proteins, immunoglobulins, and collagen types I, II, IV and V.
Format: Purified
储存及稳定性
Antibody is stable for 12 months from date of receipt when stored at 2-8ºC. Remove any nondispersed aggregates by microcentrifugation prior to use.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
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