Anti-Fibrinogen Antibody, FITC conjugated

Prior to conjugation, the antibody was specific for fibrinogen when tested against human plasma by Coomassie Blue R stained double immunodiffusion and immunoelectrophoresis. Undesired immune reactivity has been removed by solid phase immunoadsorption.

LABELLING RATIO

Fluorescein:antibody molar ratio: 2.8 - 3.3

Absorbance ratio (A493/A276): 0.78 + 0.05

Purified fibrinogen derived from pooled human plasma.

Detect Fibrinogend using this Anti-Fibrinogen Antibody, FITC conjugated validated for use in IF, ID & WB.

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

WORKING DILUTION

The final working dilution is dependent on the specific assay conditions. However, in fluorescence microscopy good results can be expected with dilutions in the following ranges:

1. Incident light excitation

Direct immunofluorescence: 1/10 - 1/80

Indirect immunofluorescence: 1/20 - 1/400

2. Transmitted light excitation

Direct immunofluorescence: 1/5 - 1/40

Indirect immunofluorescence: 1/10 - 1/80

The antibody is supplied in 20mM Tris, 70mM NaCl, pH 8.0, with 0.1% sodium azide as preservative.

Store at +2 to +8°C, for up to 6 months, undiluted. Protect from light.

WARNING: This reagent contains 0.1% sodium azide as preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.