Anti-EB1 Antibody

End binding protein 1 (EB1) is thought to be associated with adenomatous polyposis coli (APC) and may play a part in regulation of microtubule dynamics in the mammalian cells. EB1 could also play a role in the regulation of microtubule assembly and stability. EB1 is essential for proper cilia assembly in fibroblasts. EB1 has been shown to excite spontaneous nucleation and microtubule growth. APC gene mutation and the loss of negatively regulating WNT signaling is thought to play a role in the proliferation changes of cancer. The loss of association with EB1 could contribute to the development of certain cancers.

~32 kDa observed.
An uncharacterized band appears at ~52 kDa

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human EB1.

Anti-EB1 Antibody is an antibody against EB1 for use in WB & IC.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected EB1 in NIH/3T3, A431, and HeLa cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This antibody recognizes EB1 at the C-terminus.
EB1 is 67% homologous to EB3.

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected EB1 in 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.