Anti-TIMP-3 Antibody, CT

Tissue inhibitor of metalloproteinases 3 (TIMP-3) is a member of the TIMP family. The TIMPs are so named because they are slow, tight binding endogenous inhibitors of the Matrix Metalloproteinases (MMPs). The four TIMPs have different inhibition constants for the different MMPs studied. TIMPs have been shown to have activity against the ADAMs family of proteinases. TIMP-3 is an efficient "sheddase" inhibitor, inhibiting ADAM-17 (TACE) at the low nanomolar levels seen with MMP inhibition. The other ADAMs proteinases have not yet been assayed for TIMP inhibition, but TIMP-2 seems to be much less active on ADAM-17 than isTIMP-3. TIMP-3 is thought to be constitutively produced by many cell types and is inducible in others. The TIMP-3 localization differs from the other 3 TIMPs, and is thought to be primarily deposited into the extracellular matrix.

The antibody recognizes TIMP-3 at the C-terminus. Does not appear to cross react with TIMP-1 or TIMP-2.

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to human TIMP-3 at the C-terminus.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected TIMP-3 in C6 cells.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

This Anti-TIMP-3 Antibody, C-terminus is validated for use in WB, IC for the detection of TIMP-3.

Evaluated by Western Blot using an HL-60 cell lysate supplemented with PMA-conditioned media.

Western Blot Analysis: 0.5 µg/ml of this antibody detected TIMP-3 on 10 µg of HL-60 in PMA-conditioned media.

Bands may be observed for reduced protein bands at ~ 24 kDa (unglycosylated) and ~ 30 kDa (glycosylated). Additional TIMP-3 bands MAY be visible at 50 kDa (dimer), 12 kDa, and 15 kDa (breakdown products) and sample dependent.

Replaces: AB802

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HL-60 cell lysate in PMA-conditioned media.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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