产品名称
Anti-Dishevelled-2 Antibody, Chemicon®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
western blot: suitable
input
sample type hematopoietic stem cell(s)
sample type neural stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... DVL2(1856)
Application
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Hematopoietic Stem Cells
Neural Stem Cells
Hematopoietic Stem Cells
Neural Stem Cells
This Anti-Dishevelled-2 Antibody is validated for use in WB for the detection of Dishevelled-2.
Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~95kDa.
Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).
Optimal working dilutions must be determined by end user.
Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Dishevelled-2 protein (dvl-2). By Western blot the antibody shows no cross reactivity with recombinant dishevelled-1 (dvl-1) or dishevelled-3 (dvl-3) protein. Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-2 protein.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
DVL2 is a member of multigene family that also contains DVL1 and DVL3. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein.
Immunogen
Synthetic peptide corresponding to amino acids 633-645 of human disheveiled-2 protein.
Physical form
Format: Purified
Partially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide.
Preparation Note
Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
法规信息
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